Shoot control of hypernodulation and aberrant root formation in the har1-1 mutant of Lotus japonicus


Autoria(s): Jiang, Qunyi Y.; Gresshoff, Peter M.
Data(s)

01/01/2002

Resumo

The har1-1 mutant of Lotus japonicus B-129-S9 Gifu is characterized by two phenotypes: greater than normal nodulation (hypernodulation) and significantly inhibited root growth in the presence of its microsymbiont Mesorhizobium loti strain NZP2235. We demonstrate that the two traits co-segregate, suggesting a single genetic alteration involving developmental pleiotropy. A cross between the mutant and genotype Funakura (with wild-type root and nodule morphology) demonstrated Mendelian recessive segregation of both phenotypes (root and nodule) in 216 F2 individuals. Using DNA-amplification fingerprinting polymorphisms in Gifu har1-1 and Funakura, the mutant locus was positioned between two markers at about 7 and 13 cM distance. Reciprocal hypocotyl grafting of shoots and roots showed that the hypernodulation and reduced root phenotypes are both predominantly controlled by the shoot.

Identificador

http://espace.library.uq.edu.au/view/UQ:61746

Idioma(s)

eng

Publicador

CSIRO

Palavras-Chave #Plant Sciences #Genetic Mapping #Har1-1 #Nodulation #Symbiosis #Systemic Control #Dna #Supernodulation #Transformation #Ngr234 #Genes #Plant #C1 #270800 Biotechnology #620108 Grain legumes
Tipo

Journal Article