A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector
Contribuinte(s) |
H. P. Spaink |
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Data(s) |
01/03/2001
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Resumo |
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens. |
Identificador | |
Idioma(s) |
eng |
Publicador |
The American Phytopathological Society |
Palavras-Chave | #Biochemistry & Molecular Biology #Biotechnology & Applied Microbiology #Plant Sciences #Arabidopsis #Functional Genomics #Map-based Cloning #Symbiosis #Arabidopsis-thaliana #Escherichia-coli #Dna Fragments #Legume #Genes #Sequence #Mutants #Genome #Identification #Cloning #C1 #270202 Genome Structure #620108 Grain legumes |
Tipo |
Journal Article |