A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector


Autoria(s): Men, Artem E.; Meksem, Khalid; Kassem, My Abdelmajid; Lohar, Dasharath; Stiller, Jiri; Lightfoot, David; Gresshoff, Peter M.
Contribuinte(s)

H. P. Spaink

Data(s)

01/03/2001

Resumo

We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.

Identificador

http://espace.library.uq.edu.au/view/UQ:60142

Idioma(s)

eng

Publicador

The American Phytopathological Society

Palavras-Chave #Biochemistry & Molecular Biology #Biotechnology & Applied Microbiology #Plant Sciences #Arabidopsis #Functional Genomics #Map-based Cloning #Symbiosis #Arabidopsis-thaliana #Escherichia-coli #Dna Fragments #Legume #Genes #Sequence #Mutants #Genome #Identification #Cloning #C1 #270202 Genome Structure #620108 Grain legumes
Tipo

Journal Article