Role of myofibroblasts in tumour encapsulation of hepatocellular carcinoma in haemochromatosis


Autoria(s): Bridle, Kim R.; Crawford, Darrell H. G.; Powell, Lawrie W.; Ramm, Grant A.
Contribuinte(s)

Alaistair Burt

Data(s)

01/04/2001

Resumo

Background/Aims: Hepatocellular carcinoma is a carcinoma malignancy and a major complication of untreated haemochromatosis. Encapsulation of liver tumours has been associated with a better prognosis and longer disease-free periods following resection, This study investigated the source of the tumour capsule in patients with haemochromatosis and coexisting hepatocellular carcinoma and examined potential factors influencing development. Methods: Five haemochromatosis patients with encapsulated hepatocellular carcinoma were studied. Myofibroblasts were identified using combined immunohistochemistry and in situ hybridisation for a-smooth muscle actin and procollagen alpha (1)(I) mRNA, respectively. Immunohistochemistry was also performed for transforming growth factor (TGF)-beta (1), platelet-derived growth factor (PDGF)-beta receptor and malondialdehyde. Results. Procollagen alpha (1)(I) mRNA co-localised to alpha -smooth muscle actin positive myofibroblasts. The number of myofibroblasts was maximal within the capsule and decreased away from the tumour. TGF-beta (1) protein was expressed in iron-loaded cells in non-tumour liver at the interface of tumour capsule. PDGF-beta receptor expression was observed in mesenchymal cells in the tumour capsule and in portal tracts. Malondialdehyde adducts were observed in the tumour, non-tumour tissue and in the capsule. Conclusions: This study provides evidence that myofibroblasts are the cell type responsible for collagen production within the tumour capsule surrounding hepatocellular carcinoma in haemochromatosis, The production of TGF-beta (1) by iron-loaded hepatic cells at the tumour capsule interface may perpetuate the myofibroblastic phenotype, resulting in, the formation of the tumour capsule.

Identificador

http://espace.library.uq.edu.au/view/UQ:59804

Idioma(s)

eng

Publicador

Blackwell Munksgaard

Palavras-Chave #Gastroenterology & Hepatology #Alpha-smooth Muscle Actin #Collagen #Tgf-beta #Malondialdehyde #Pdgf-beta Receptor #Hepatic Stellate Cells #Myofibroblasts #Hepatocellular Carcinoma #Haemochromatosis #Growth-factor Receptor #Genetic Hemochromatosis #Tissue Inhibitor #Oxidative Stress #Lung-cancer #Liver #Iron #Expression #Activation #C1 #321006 Gastroenterology and Hepatology #730113 Digestive system and disorders
Tipo

Journal Article