FAM deubiquitylating enzyme is essential for preimplantation mouse embryo development


Autoria(s): Pantaleon, M.; Kanai-Azuma, M.; Mattick, J. S.; Kaibuchi, K.; Kaye, P. L.; Wood, S. A.
Data(s)

01/01/2001

Resumo

FAM is a developmentally regulated substrate-specific deubiquitylating enzyme. It binds the cell adhesion and signalling molecules beta -catenin and A-F-6 in vitro, and stabilises both in mammalian cell culture. To determine if FAM is required at the earliest stages of mouse development we examined its expression and function in preimplantation mouse embryos. FAM is expressed at all stages of preimplantation development from ovulation to implantation. Exposure of two-cell embryos to FAM-specific antisense, but not sense, oligodeoxynucleotides resulted in depletion of the FAM protein and failure Of the embryos to develop to blastocysts. Loss of FAM had two physiological effects, namely, a decrease in cleavage rate and an inhibition of cell adhesive events. Depletion of FAM protein was mirrored by a loss of beta -catenin such that very little of either protein remained following 72 h culture. The residual beta -catenin was localised to sites of cell-cell contact suggesting that the cytoplasmic pool of beta -catenin is stabilised by FAM. Although AF-6 levels initially decreased they returned to normal. However, the nascent protein was mislocalised at the apical surface of blastomeres. Therefore FAM is required for preimplantation mouse embryo development and regulates beta -catenin and AF-6 in vivo. (C) 2001 Elsevier Science Ireland Lid. All rights reserved.

Identificador

http://espace.library.uq.edu.au/view/UQ:59631

Idioma(s)

eng

Publicador

Elsevier Science Ltd

Palavras-Chave #Developmental Biology #Deubiquitylating #Beta-catenin #Af-6 #Cell Adhesion #Blastocyst #Compaction #Blastomere Cleavage #Ubiquitin #Facets Deubiquitinating Enzyme #Drosophila Eye Development #Cell-cell Junctions #Ras Target Af-6 #Cytoplasmic Domain #Tight Junctions #Protein Af-6 #E-cadherin #Embryogenesis #C1 #270205 Genetic Development (incl. Sex Determination) #780105 Biological sciences
Tipo

Journal Article