GABA(A) receptor alpha-subunit proteins in human chronic alcoholics


Autoria(s): Lewohl, J. M.; Huygens, F.; Cranet, D. I.; Dodd, P. R.
Data(s)

01/01/2001

Resumo

Antibodies were raised against specific peptides from N-terminal regions of the alpha (1) and alpha (3) isoforms of the GABA(A) receptor, and used to assess the relative expression of these proteins in the superior frontal and primary motor cortices of 10 control, nine uncomplicated alcoholic and six cirrhotic alcoholic cases were matched for age and post-mortem delay. The regression of expression on post-mortem delay was not statistically significant for either isoform in either region. In both cortical areas, the regression of a, expression on age differed significantly between alcoholic cases, which showed a decrease, and normal controls, which did not. Age had no effect on alpha (3) expression. The alpha (1) and alpha (3) isoforms were found to be expressed differentially across cortical regions and showed a tendency to be expressed differentially across case groups. In cirrhotic alcoholics, alpha (1) expression was greater in superior frontal than in motor cortex, whereas this regional difference was not significant in controls or uncomplicated alcoholics. In uncomplicated alcoholics, alpha (3) expression was significantly lower in superior frontal than in motor cortex. Expression of alpha (1) was significantly different from that Of alpha (3) in the superior frontal cortex of alcoholics, but not in controls. In motor cortex, there were no significant differences in expression between the isoforms in any case group.

Identificador

http://espace.library.uq.edu.au/view/UQ:59140

Idioma(s)

eng

Publicador

Blackwell Science

Palavras-Chave #Biochemistry & Molecular Biology #Neurosciences #Anti-peptide Antibodies #Brain Damage #Cerebral Cortex #Pathogenesis #Receptor Isoforms #Western Blotting #Rat Cerebral-cortex #Benzodiazepine Receptors #Messenger-rnas #Frontal-cortex #Binding-sites #Brain #Expression #Encephalopathy #Regions #Alters #C1 #270107 Cell Neurochemistry #270201 Gene Expression #320702 Central Nervous System #321020 Pathology #730104 Nervous system and disorders #730205 Substance abuse
Tipo

Journal Article