Properties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias
Data(s) |
01/01/2001
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Resumo |
KCNQ1 (K(V)LQT1) K+ channels play an important role during electrolyte secretion in airways and colon. KCNQ1 was cloned recently from NaCl-secreting shark rectal glands. Here we study. the properties and regulation of the cloned sK(V)LQT1 expressed in Xenopus oocytes and Chinese hamster ovary (CHO) cells and compare the results with those obtained from in vitro perfused rectal gland tubules (RGT). The expression of sKCNQ1 induced voltage-dependent, delayed activated K+ currents, which were augmented by an increase in intracellular cAMP and Ca2+. The chromanol derivatives 293B and 526B potently inhibited sKCNQ1 expressed in oocytes and CHO cells, but had little effect on RGT electrolyte transport. Short-circuit currents in RGT were activated by alkalinization and were decreased by acidification. In CHO cells an alkaline pH activated and an acidic pH inhibited 293B-sensitive KCNQ1 currents. Noise analysis of the cell-attached basolateral membrane of RGT indicated the presence of low-conductance ( |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer-Verlag |
Palavras-Chave | #Physiology #293b #Camp #Cl- Secretion #Epithelial Transport #Kcnq1 #Kvlqt1 #Squalus Acanthias #Mediated Cl Secretion #I-ks #Potassium Channel #Basolateral Membrane #Nacl Secretion #Small-conductance #Wild-type #Tubules #K(v)lqt1 #Cells #C1 #270104 Membrane Biology #730110 Respiratory system and diseases (incl. asthma) #1103 Clinical Sciences |
Tipo |
Journal Article |