Patients with oral squamous cell carcinoma are characterized by increased frequency of suppressive regulatory T cells in the blood and tumor microenvironment


Autoria(s): GASPAROTO, Thais Helena; MALASPINA, Tatiana Salles de Souza; BENEVIDES, Luciana; MELO JR., Edgard Jose Franco de; COSTA, Maria Renata Sales Nogueira; DAMANTE, Jose Humberto; IKOMA, Maura Rosane Valerio; GARLET, Gustavo Pompermaier; CAVASSANI, Karen Angelica; SILVA, Joao Santana da; CAMPANELLI, Ana Paula
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Oral squamous cell carcinoma (OSCC) is a cancerous lesion with high incidence worldwide. The immunoregulatory events leading to OSCC persistence remain to be elucidated. Our hypothesis is that regulatory T cells (Tregs) are important to obstruct antitumor immune responses in patients with OSCC. In the present study, we investigated the frequency, phenotype, and activity of Tregs from blood and lesions of patients with OSCC. Our data showed that > 80% of CD4(+)CD25(+) T cells isolated from PBMC and tumor sites express FoxP3. Also, these cells express surface Treg markers, such as GITR, CD45RO, CD69, LAP, CTLA-4, CCR4, and IL-10. Purified CD4(+)CD25(+) T cells exhibited stronger suppressive activity inhibiting allogeneic T-cell proliferation and IFN-gamma production when compared with CD4(+)CD25(+) T cells isolated from healthy individuals. Interestingly, approximately 25% of CD4(+)CD25(-) T cells of PBMC from patients also expressed FoxP3 and, although these cells weakly suppress allogeneic T cells proliferative response, they inhibited IFN-gamma and induced IL-10 and TGF-beta secretion in these co-cultures. Thus, our data show that Treg cells are present in OSCC lesions and PBMC, and these cells appear to suppress immune responses both systemically and in the tumor microenvironment.

State of Sao Paulo Research Foundation (FAPESP)[06/04264-9]

Identificador

CANCER IMMUNOLOGY IMMUNOTHERAPY, v.59, n.6, p.819-828, 2010

0340-7004

http://producao.usp.br/handle/BDPI/25839

10.1007/s00262-009-0803-7

http://dx.doi.org/10.1007/s00262-009-0803-7

Idioma(s)

eng

Publicador

SPRINGER

Relação

Cancer Immunology Immunotherapy

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #T regulatory cells #OSCC #Immunosuppression #PERIPHERAL-BLOOD #IMMUNOLOGICAL-TOLERANCE #CANCER-PATIENTS #BREAST-CANCER #IMMUNOTHERAPY #LYMPHOCYTES #PREVALENCE #CONVERSION #RESPONSES #IMMUNITY #Oncology #Immunology
Tipo

article

original article

publishedVersion