Effects of Ethanol on Synthesis of Prostaglandin F2 alpha in Bovine Females


Autoria(s): BARROS, F. R. O. de; BERTAN, C. M.; DIAS, L. O. P.; FANTINI, D. A.; AYRES, G. R.; MARQUES, V. B.; MIGUEZ, P. H. P.; BINELLI, M.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Ethanol stimulates the production of prostaglandins in many species. The purpose of this study was to verify the effect of ethanol on the production of prostaglandin F2 alpha (PGF2 alpha) and luteolysis in bovine females. In the first experiment, Holstein cows at day 17 of the oestrous cycle were treated with 100% ethanol (0.05 ml/kg of body weight, IV; n = 5), saline (0.05 ml/kg of body weight, IV; n = 4) or synthetic prostaglandin (150 mu g of D-cloprostenol/cow, IM; n = 4). The plasma concentrations of 13, 14-dihydro-15-keto PGF2 alpha (PGFM; the main metabolite of PGF2 alpha measured in the peripheral blood) were assessed by radioimmunoassay (RIA). There was an acute release of PGFM in response to ethanol comparing to other treatments (p <= 0.05). However, only cows treated with PGF2 alpha underwent luteolysis. In the second experiment, endometrial explants of cross-bred beef cows (n = 4) slaughtered at day 17 of the oestrous cycle were cultured for 4 h. During the last 3 h, the explants were cultured with medium supplemented with 0, 0.1, I, 10 or 100 mu l of 100% ethanol/ml. Medium samples were collected at hours 1 and 4 and concentrations of PGF2 alpha were measured by RIA. Ethanol did not induce PGF2 alpha production by the endometrium. In conclusion, ethanol does not cause luteolysis in cows because it stimulates production of PGF2 alpha in extra-endometrial tissues.

FAPESP

Identificador

REPRODUCTION IN DOMESTIC ANIMALS, v.45, n.5, p.846-850, 2010

0936-6768

http://producao.usp.br/handle/BDPI/25494

10.1111/j.1439-0531.2009.01367.x

http://dx.doi.org/10.1111/j.1439-0531.2009.01367.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL PUBLISHING, INC

Relação

Reproduction in Domestic Animals

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL PUBLISHING, INC

Palavras-Chave #INTERFERON-TAU #CORPUS-LUTEUM #FATTY-ACIDS #DAIRY-COWS #CATTLE #SYNCHRONIZATION #ENDOMETRIUM #SECRETION #ESTRUS #CELLS #Agriculture, Dairy & Animal Science #Reproductive Biology #Veterinary Sciences
Tipo

article

original article

publishedVersion