Evidence of the presence of T helper type 17 cells in chronic lesions of human periodontal disease
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2009
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Resumo |
Periodontal disease is a chronic inflammation of the attachment structures of the teeth, triggered by potentially hazardous microorganisms and the consequent immune-inflammatory responses. In humans, the T helper type 17 (Th17) lineage, characterized by interleukin-17 (IL-17) production, develops under transforming growth factor-beta (TGF-beta), IL-1 beta, and IL-6 signaling, while its pool is maintained by IL-23. Although this subset of cells has been implicated in various autoimmune, inflammatory, and bone-destructive conditions, the exact role of T lymphocytes in chronic periodontitis is still controversial. Therefore, in this study we investigated the presence of Th17 cells in human periodontal disease. Gingival and alveolar bone samples from healthy patients and patients with chronic periodontitis were collected and used for the subsequent assays. The messenger RNA expression for the cytokines IL-17, TGF-beta, IL-1 beta, IL-6, and IL-23 in gingiva or IL-17 and receptor activator for nuclear factor-kappa B ligand in alveolar bone was evaluated by real-time polymerase chain reaction. The production of IL-17, TGF-beta, IL-1 beta, IL-6, and IL-23 proteins was evaluated by immunohistochemistry and the presence of Th17 cells in the inflamed gingiva was confirmed by immunofluorescence confocal microscopy for CD4 and IL-17 colocalization. Our data demonstrated elevated levels of IL-17, TGF-beta, IL-1 beta, IL-6, and IL-23 messenger RNA and protein in diseased tissues as well as the presence of Th17 cells in gingiva from patients with periodontitis. Moreover, IL-17 and the bone resorption factor RANKL were abundantly expressed in the alveolar bone of diseased patients, in contrast to low detection in controls. These results provided strong evidence for the presence of Th17 cells in the sites of chronic inflammation in human periodontal disease. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) |
Identificador |
ORAL MICROBIOLOGY AND IMMUNOLOGY, v.24, n.1, p.1-6, 2009 0902-0055 http://producao.usp.br/handle/BDPI/24948 10.1111/j.1399-302X.2008.00463.x |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL PUBLISHING, INC |
Relação |
Oral Microbiology and Immunology |
Direitos |
restrictedAccess Copyright WILEY-BLACKWELL PUBLISHING, INC |
Palavras-Chave | #immune response #interleukin-17 #periodontal disease #receptor activator of nuclear factor-kappa B ligand #T helper type 17 #EPSTEIN-BARR-VIRUS #ACTINOBACILLUS-ACTINOMYCETEMCOMITANS #PHYSIOLOGICAL INHIBITORS #CYTOKINE PROFILES #BONE DESTRUCTION #CUTTING EDGE #IL-17 #RECEPTOR #TISSUES #INTERLEUKIN-17 #Dentistry, Oral Surgery & Medicine #Immunology #Microbiology |
Tipo |
article original article publishedVersion |