Molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli from Rio de Janeiro, Brazil


Autoria(s): PEIRANO, G.; ASENSI, M. D.; PITONDO-SILVA, A.; PITOUT, J. D. D.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

Twenty-five extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli clinical isolates from Rio de Janeiro, Brazil were characterized by isoelectric focusing, PCR and sequencing of bla(ESBL) genes, plasmid-mediated quinolone resistance determinants, phylogenetic groups, replicon typing, pulsed-field electrophoresis, and multilocus sequencing typing. Twenty-three (92%) ESBL-producing E. coli isolates were positive for bla(CTX-M) genes, aac(6`)-lb-cr, and qnrB. Genetic relatedness of ESBL producers clustered seven (28%) CTX-M-15-producing isolates as sequence type (ST) 410, clonal complex (CC) 23, and two (8%) as clone O25-ST131. Our results illustrate the predominance of phylo-group A (52%), ST410 (CC 23) and CTX-M-15 among ESBL-producing E. coli isolates from hospitals in Rio de Janeiro.

Calgary Laboratory Services[73-4063]

Merck (Canada)

Merck

Astra Zeneca

Identificador

CLINICAL MICROBIOLOGY AND INFECTION, v.17, n.7, p.1039-1043, 2011

1198-743X

http://producao.usp.br/handle/BDPI/24935

10.1111/j.1469-0691.2010.03440.x

http://dx.doi.org/10.1111/j.1469-0691.2010.03440.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

Relação

Clinical Microbiology and Infection

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #Brazil #CTX-M-15 #Escherichia coli #phylogroup A #ST410 #CALGARY HEALTH REGION #ENTEROBACTERIACEAE #EMERGENCE #EPIDEMIOLOGY #COMMUNITY #CLONE #Infectious Diseases #Microbiology
Tipo

article

original article

publishedVersion