MAPK and angiotensin II receptor in kidney of newborn rats from losartan-treated dams


Autoria(s): BALBI, Ana Paula Coelho; MARIN, Evelyn Cristina Santana; FRANCESCATO, Heloisa Della Coletta; COSTA, Roberto Silva; COIMBRA, Terezila Machado
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Several lines of evidence suggest that angiotensin II (A-II) participates in the postnatal development of the kidney in rats. Many effects of A-II are mediated by mitogen-activated protein kinase (MAPK) pathways. This study investigated the influence that treatment with losartan during lactation has on MAPKs and on A-II receptor types 1 (AT(1)) and 2 (AT(2)) expression in the renal cortices of the offspring of dams exposed to losartan during lactation. In addition, we evaluated the relationship between such expression and changes in renal function and structure. Rat pups from dams receiving 2% sucrose or losartan diluted in 2% sucrose (40 mg/dl) during lactation were killed 30 days after birth, and the kidneys were removed for histological, immunohistochemical, and Western blot analysis. AT(1) and AT(2) receptors and p-p38, c-Jun N-terminal kinases (p-JNK) and extracellular signal-regulated protein kinases (p-ERK) expression were evaluated using Western blot analysis. The study-group rats presented an increase in AT(2) receptor and MAPK expression. In addition, these rats also presented lower glomerular filtration rate (GFR), greater albuminuria, and changes in renal structure. In conclusion, newborn rats from dams exposed to losartan during lactation presented changes in renal structure and function, which were associated with AT(2) receptor and MAPK expression in the kidneys.

Identificador

PEDIATRIC NEPHROLOGY, v.23, n.9, p.1433-1444, 2008

0931-041X

http://producao.usp.br/handle/BDPI/24438

10.1007/s00467-008-0830-1

http://dx.doi.org/10.1007/s00467-008-0830-1

Idioma(s)

eng

Publicador

SPRINGER

Relação

Pediatric Nephrology

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #renal development #angiotensin II #MAPK #angiotensin II receptor #losartan #lactation #PROTEIN-KINASE FAMILY #SMOOTH-MUSCLE CELLS #EXTRACELLULAR-MATRIX #DEVELOPING GLOMERULI #MYOCARDIAL-ISCHEMIA #GROWTH-FACTOR #EXPRESSION #INHIBITION #RENIN #REPERFUSION #Pediatrics #Urology & Nephrology
Tipo

article

original article

publishedVersion