Neutrophil migration induced by IL-1 beta depends upon LTB4 released by macrophages and upon TNF-alpha and IL-1 beta released by mast cells


Autoria(s): OLIVEIRA, S. H. P.; CANETTI, C.; RIBEIRO, R. A.; CUNHA, F. Q.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

In the present study, we investigate whether mast cells and macrophages are involved in the control of IL-1 beta-induced neutrophil migration, as well as the participation of chemotactic mediators. IL-1 beta induced a dose-dependent neutrophil migration to the peritoneal cavity of rats which depends on LTB4, PAF and cytokines, since the animal treatment with inhibitors of these mediators (MK 886, PCA 4248 and dexamethasone respectively) inhibited IL-1 beta-induced neutrophil migration. The neutrophil migration induced by IL-1 beta is dependent on mast cells and macrophages, since depletion of mast cells reduced the process whereas the increase of macrophage population enhanced the migration. Moreover, mast cells or macrophages stimulated with IL-1 beta released a neutrophil chemotactic factor, which mimicked the neutrophil migration induced by IL-1 beta. The chemotactic activity of the supernatant of IL-1 beta-stimulated macrophages is due to the presence of LTB4, since MK 886 inhibited its release. Moreover, the chemotactic activity of IL-1 beta-stimulated mast cells supernatant is due to the presence of IL-1 beta and TNF-alpha, since antibodies against these cytokines inhibited its activity. Furthermore, significant amounts of these cytokines were detected in the supernatant. In conclusion, our results suggest that neutrophil migration induced by IL-1 beta depends upon LTB4 released by macrophages and upon IL-1 beta and TNF alpha released by mast cells.

Identificador

INFLAMMATION, v.31, n.1, p.36-46, 2008

0360-3997

http://producao.usp.br/handle/BDPI/24271

10.1007/s10753-007-9047-x

http://dx.doi.org/10.1007/s10753-007-9047-x

Idioma(s)

eng

Publicador

SPRINGER/PLENUM PUBLISHERS

Relação

Inflammation

Direitos

restrictedAccess

Copyright SPRINGER/PLENUM PUBLISHERS

Palavras-Chave #IL-1 beta #LTB4 #mast cells #neutrophil migration #TNF-alpha #NECROSIS-FACTOR-ALPHA #RECEPTOR ANTAGONIST #ENDOTHELIAL-CELLS #RHEUMATOID-ARTHRITIS #INTERLEUKIN-1 LEVELS #LEUKOCYTE MIGRATION #LEUKOTRIENE B-4 #RAT PERITONEAL #IN-VITRO #INFLAMMATION #Cell Biology #Immunology
Tipo

article

original article

publishedVersion