Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
19/10/2012
19/10/2012
2011
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| Resumo |
293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially located in gene regions. The integrations in both cell lines were distant from the CpG islands and from the transcription start sites. A comparison between the two cell lines showed that the lentiviral-transduced DNA had the same preferred regions in the two different cell lines. CNPq (Conselho Nacional de Desenvolvimento Tecnologico) FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) |
| Identificador |
BIOTECHNOLOGY LETTERS, v.33, n.1, p.23-31, 2011 0141-5492 http://producao.usp.br/handle/BDPI/24106 10.1007/s10529-010-0387-5 |
| Idioma(s) |
eng |
| Publicador |
SPRINGER |
| Relação |
Biotechnology Letters |
| Direitos |
restrictedAccess Copyright SPRINGER |
| Palavras-Chave | #Coagulation factor VIII #Lentiviral vector #Integration sites #SEVERE COMBINED IMMUNODEFICIENCY #RETROVIRAL GENE MARKING #REPOPULATING CELLS #HUMAN GENOME #SITES #THERAPY #PROMOTER #SEQUENCE #Biotechnology & Applied Microbiology |
| Tipo |
article original article publishedVersion |
