Frequency and prognostic relevance of cancer testis antigen 45 expression in multiple myeloma


Autoria(s): ANDRADE, Valeria C. C.; VETTORE, Andre L.; SILVA, Maria Regina Regis; FELIX, Roberta S.; ALMEIDA, Manuella S. S.; CARVALHO, Fabricio de; ZAGO, Marco Antonio; CABALLERO, Otavia L.; SIMPSON, Andrew J.; COLLEONI, Gisele W. B.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

Objective. This study aims to analyze the expression of cancer testis antigen 45 (CT45) in normal tissues and in plasma cell disorders and to identify possible associations with clinical data and prognosis in multiple myeloma (MM) patients. Materials and Methods. Expression of CT45 was studied in 20 normal tissues (testis, placenta, skeletal muscle, bladder, lung, spleen, heart, brain and fetal brain, thymus, uterus, stomach, mammary gland, pancreas, prostate, small intestine, kidney, adrenal gland, spinal cord, colon, and one pool of 10 normal bone marrow samples) and bone marrow aspirates from 3 monoclonal gammopathies of undetermined significance, 5 solitary plasmacytomas, 61 newly diagnosed MM patients and MM cell line U266 by reverse transcriptase polymerase chain reaction. Results. CT45 was positive in 3 of 20 (15%) normal tissues tested: lung, brain (both fetal and adult), and spinal cord. Among monoclonal gammopathies, CT45 was positive in 2 of 5 (40%) solitary plasmacytomas bone marrow aspirates, 10 of 61 (16%) MM bone marrow aspirates, and in the U266 MM cell line. Conclusions. We did not find associations between bone marrow histology and CT45 expression. However, we demonstrated for the first time that positive expression of CT45 was associated with poor prognostic (international Staging System) and poor outcomes in MM patients, meaning that CT45-positive cases presented seven times more chance of worse evolution than the negative ones. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Sao Paulo, Brazil[04/13213-3]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Sao Paulo, Brazil[04/12855-1]

Identificador

EXPERIMENTAL HEMATOLOGY, v.37, n.4, p.446-449, 2009

0301-472X

http://producao.usp.br/handle/BDPI/23978

10.1016/j.exphem.2008.12.003

http://dx.doi.org/10.1016/j.exphem.2008.12.003

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE INC

Relação

Experimental Hematology

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE INC

Palavras-Chave #GENES #Hematology #Medicine, Research & Experimental
Tipo

article

original article

publishedVersion