Apoptosis induction by (+)alpha-tocopheryl succinate in the absence or presence of all-trans retinoic acid and arsenic trioxide in NB4, NB4-R2 and primary APL cells
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2009
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Resumo |
We analyzed the effect of (+)alpha-tocopheryl succinate (alpha-TOS) alone or associated with arsenic trioxide (ATO) or all-trans retinoid acid (ATRA) in acute promyelocytic leukemia (APL). alpha-TOS-induced apoptosis in APL clinical samples and in ATRA-sensitive (NB4) and ATRA-resistant (NB4-R2) APL cell lines. The effective dose 50% (ED-50) was calculated to be 71 and 58 mu M, for NB4 and NB4-R2, respectively. a-TOS neither induced nor modified ATRA-induced differentiation of APL cells, and did not affect the proliferation and differentiation of normal CD34(+) hematopoietic progenitors in methylcellulose assays. alpha-TOS exerted a moderate antagonistic effect to ATO-induced apoptosis when treatment was done simultaneously but when alpha-TOS was added 24 h after ATO, an additive effect was observed. Our results support the concept of alpha-TOS as an anti-leukemic compound which spares normal hematopoiesis. (C) 2008 Elsevier Ltd. All rights reserved. Conselho Nacional de Desenvolvirnento Cientifico e Tecnologico (CNPq)[481911/2004-9] FAPESP[1998/14247-6] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[2001/12406-4] |
Identificador |
LEUKEMIA RESEARCH, v.33, n.7, p.958-963, 2009 0145-2126 http://producao.usp.br/handle/BDPI/23964 10.1016/j.leukres.2008.09.035 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD |
Relação |
Leukemia Research |
Direitos |
closedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #Acute promyelocytic leukemia #alpha-tocopheryl succinate #Arsenic trioxide #Apoptosis #ACUTE PROMYELOCYTIC LEUKEMIA #VITAMIN-E-SUCCINATE #CANCER CELLS #COMBINATION #THERAPY #GROWTH #AGENT #MICE #CHEMOTHERAPY #RESISTANCE #Oncology #Hematology |
Tipo |
article original article publishedVersion |