Apoptosis induction by (+)alpha-tocopheryl succinate in the absence or presence of all-trans retinoic acid and arsenic trioxide in NB4, NB4-R2 and primary APL cells


Autoria(s): FREITAS, Rosana Aparecida; SANTOS, Guilherme Augusto Silva dos; TEIXEIRA, Hamilton Luiz Gimenes; SCHEUCHER, Priscila Santos; LUCENA-ARAUJO, Antonio Roberto; LIMA, Ana Silvia Gouveia; LIMA, Rodrigo Siqueira Abreu e; GARCIA, Aglair Bergamo; JORDAO JR., Alceu Afonso; FAICAO, Roberto Passetto; VANNUCCHI, Helio; REGO, Eduardo Magalhaes
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

We analyzed the effect of (+)alpha-tocopheryl succinate (alpha-TOS) alone or associated with arsenic trioxide (ATO) or all-trans retinoid acid (ATRA) in acute promyelocytic leukemia (APL). alpha-TOS-induced apoptosis in APL clinical samples and in ATRA-sensitive (NB4) and ATRA-resistant (NB4-R2) APL cell lines. The effective dose 50% (ED-50) was calculated to be 71 and 58 mu M, for NB4 and NB4-R2, respectively. a-TOS neither induced nor modified ATRA-induced differentiation of APL cells, and did not affect the proliferation and differentiation of normal CD34(+) hematopoietic progenitors in methylcellulose assays. alpha-TOS exerted a moderate antagonistic effect to ATO-induced apoptosis when treatment was done simultaneously but when alpha-TOS was added 24 h after ATO, an additive effect was observed. Our results support the concept of alpha-TOS as an anti-leukemic compound which spares normal hematopoiesis. (C) 2008 Elsevier Ltd. All rights reserved.

Conselho Nacional de Desenvolvirnento Cientifico e Tecnologico (CNPq)[481911/2004-9]

FAPESP[1998/14247-6]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[2001/12406-4]

Identificador

LEUKEMIA RESEARCH, v.33, n.7, p.958-963, 2009

0145-2126

http://producao.usp.br/handle/BDPI/23964

10.1016/j.leukres.2008.09.035

http://dx.doi.org/10.1016/j.leukres.2008.09.035

Idioma(s)

eng

Publicador

PERGAMON-ELSEVIER SCIENCE LTD

Relação

Leukemia Research

Direitos

closedAccess

Copyright PERGAMON-ELSEVIER SCIENCE LTD

Palavras-Chave #Acute promyelocytic leukemia #alpha-tocopheryl succinate #Arsenic trioxide #Apoptosis #ACUTE PROMYELOCYTIC LEUKEMIA #VITAMIN-E-SUCCINATE #CANCER CELLS #COMBINATION #THERAPY #GROWTH #AGENT #MICE #CHEMOTHERAPY #RESISTANCE #Oncology #Hematology
Tipo

article

original article

publishedVersion