The Protective Effect of CAPE on Hepatic Ischemia/Reperfusion Injury in Rats


Autoria(s): SAAVEDRA-LOPES, Milena; RAMALHO, Fernando S.; RAMALHO, Leandra N. Z.; ANDRADE-SILVA, Alessandra; MARTINELLI, Ana L. C.; JORDAO JR., Alceu A.; CASTRO-E-SILVA, Orlando; ZUCOLOTO, Sergio
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background/Aims. The transcription factor nuclear factor-kappa B (NF-kappa B) exerts a pivotal role in the pathogenesis of hepatic ischemia/reperfusion (I/R) injury. Caffeic acid phenyl ester (CAPE), a potent and specific NF-kappa B inhibitor, presents protective effects on I/R injury in some tissues. This study aimed to evaluate the effect of CAPE on hepatic I/R injury in rats. Materials and methods. Wistar rats were submitted to a sham operation, 60 min ischemia, or 60 min ischemia plus saline or CAPE treatment followed by 6 h reperfusion. Liver tissue injury was evaluated by alanine aminotransferase, aspartate aminotransferase, and tissue glutathione measurement, and histological damage score. Apoptotic hepatocytes were determined by the transferase-mediated dUTP-biotin nick-end labeling assay. Hepatic neutrophil accumulation was assessed by the naphthol method. Lipid peroxidation and NF-kappa B activation were evaluated by 4-hydroxynonenal and NF-kappa B p65 immunohistochemistry, respectively. Results. Animals submitted to ischemia showed a marked increase of alanine aminotransferase and aspartate aminotransferase after reperfusion, but with lower levels in CAPE group. Tissue glutathione content declined gradually during ischemia to reperfusion and was partially recovered with CAPE treatment. The histological damage score, apoptosis index, and neutrophil infiltration, as well as 4-hydroxynonenal and NF-kappa B p65 nuclear labeling, were higher in the liver of animals submitted to I/R compared to the ischemia group. However, the CAPE treatment significantly reduced all of these alterations. Conclusions. CAPE was able to protect the liver against normothermic I/R injury in rats. This effect may be associated with the inhibition of the NF-kappa B signaling pathway and decrease of the acute inflammatory response following I/R in the liver. (C) 2008 Elsevier Inc. All rights reserved.

FAEPA

CAPES

CNPq

Identificador

JOURNAL OF SURGICAL RESEARCH, v.150, n.2, p.271-277, 2008

0022-4804

http://producao.usp.br/handle/BDPI/23906

10.1016/j.jss.2008.01.039

http://dx.doi.org/10.1016/j.jss.2008.01.039

Idioma(s)

eng

Publicador

ACADEMIC PRESS INC ELSEVIER SCIENCE

Relação

Journal of Surgical Research

Direitos

restrictedAccess

Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE

Palavras-Chave #liver #I/R injury #CAPE #NF-kappa B #ACID PHENETHYL ESTER #ISCHEMIA-REPERFUSION INJURY #FACTOR-KAPPA-B #INDUCED OXIDATIVE DAMAGE #NUCLEAR-FACTOR #EPITHELIAL-CELLS #LIVER #STRESS #ACTIVATION #TRANSPLANTATION #Surgery
Tipo

article

original article

publishedVersion