The effects of human herpesvirus 8 infection and interferon-gamma response in cutaneous lesions of Kaposi sarcoma differ among human immunodeficiency virus-infected and uninfected individuals


Autoria(s): GUEDES, F.; ANDRADE JR., H. F. de; FERNANDES, E. R.; TUON, F. F.; BRASIL, R. A.; PAGLIARI, C.; DUARTE, M. I. S.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background Kaposi sarcoma (KS) is associated with human herpesvirus 8 (HHV-8). The cutaneous immune response in this tumour is not well established and a better understanding is necessary. Objectives To evaluate the HHV-8 expression and immune response in cutaneous lesions of classic KS (CKS) and AIDS-associated KS (AIDS-KS). Methods We performed a quantitative immunohistochemical study of cells expressing HHV-8 latency-associated nuclear antigen (LANA), CD4, CD8 and interferon (IFN)-gamma in skin lesions from patients with CKS and AIDS-KS (with or without highly active antiretroviral therapy, HAART). Results CKS showed higher LANA expression compared with AIDS-KS, regardless of HAART. We also found higher LANA expression in nodules compared with patch/plaque lesions. The tissue CD4+ cell proportion was lower in AIDS-KS patients without HAART than in patients with CKS. In CKS lesions, CD4+ and CD8+ cells expressed IFN-gamma, as shown by double immunostaining. AIDS-KS presented low numbers of IFN-gamma-expressing cells. CD8+ cell numbers were similar in all groups, which appeared unrelated to the clinical or epidemiological type of KS. Conclusions. Our quantitative data on the pattern of KS lesions in selected groups of patients, as shown by in situ immune response, demonstrated a CD4+ T-cell involvement associated with IFN-gamma, an environment of immune response-modified human immunodeficiency virus (HIV) infection. In our sample, the promotion of KS in patients without HIV appears to be related to higher HHV-8 load or virulence than in those with AIDS. This higher resistance may be explained by a sustained immune response against this herpesvirus, that is only partially restored but effective after HAART.

Identificador

BRITISH JOURNAL OF DERMATOLOGY, v.159, n.4, p.839-846, 2008

0007-0963

http://producao.usp.br/handle/BDPI/23463

10.1111/j.1365-2133.2008.08755.x

http://dx.doi.org/10.1111/j.1365-2133.2008.08755.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

Relação

British Journal of Dermatology

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #highly active antiretroviral therapy #human herpesvirus 8 #immune response #immunohistochemistry #Kaposi sarcoma #ACTIVE ANTIRETROVIRAL THERAPY #HHV8 ASSOCIATED DISEASES #CLINICAL MANIFESTATIONS #IMMUNE RECONSTITUTION #CASTLEMANS-DISEASE #EFFUSION LYMPHOMA #HERPESVIRUS LOAD #SPINDLE CELLS #T-CELLS #AIDS #Dermatology
Tipo

article

original article

publishedVersion