Expression profiles of the glucose-dependent insulinotropic peptide receptor and LHCGR in sporadic adrenocortical tumors


Autoria(s): COSTA, Marcia Helena Soares; LATRONICO, Ana Claudia; MARTIN, Regina Matsunaga; BARBOSA, Angela S.; ALMEIDA, Madson Q.; LOTFI, Claudimara Ferini Pacicco; VALASSI, Helena P. Lima; NISHI, Mirian Yumie; LUCON, Antonio Marmo; SIQUEIRA, Sheila Aparecida; ZERBINI, Maria Claudia Nogueira; CARVALHO, Luciani Renata; MENDONCA, Berenice Bilharinho; FRAGOSO, Maria Candida Barisson Villares
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

Glucose-dependent insulinotropic peptide receptor (GIPR) and LHCGR are G-protein-coupled receptors with a wide tissue expression pattern. Aberrant expression of these receptors has rarely been demonstrated in adult sporadic adrenocortical tumors with a lack of data on pediatric tumors. We quantified the GIPR and LHCGR expression in a large cohort of 55 patients (25 children and 30 adults) with functioning and non-functioning sporadic adrenocortical tumors. Thirty-eight tumors were classified as adenomas whereas 17 were carcinomas. GIPR, and LHCGR expression were analyzed by real-time PCR and normal human pancreatic and testicular tissue samples were used as positive controls. Mean expression values were determined by fold increase in comparison with a normal adrenal pool. GIPR mRNA levels were significantly higher in adrenocortical carcinomas than in adenomas from both pediatric and adult groups. LHCGR expression was similar in both carcinomas and adenomas from the pediatric group but significantly lower in carcinomas than in adenomas from the adult group (median 0.06 and 2.3 respectively, P<0.001). GIPR was detected by immunohistochemistry in both pediatric and adult tumors. Staining and real-time PCR results correlated positively only when GIPR in RN A levels were increased at least two-fold in comparison with normal adrenal expression levels. In Conclusion, GIPR overexpression was observed in pediatric and adult adrenocortical tumors and very low levels of LHCGR expression were found in all adult adrenocortical carcinomas.

Identificador

JOURNAL OF ENDOCRINOLOGY, v.200, n.2, p.167-175, 2009

0022-0795

http://producao.usp.br/handle/BDPI/23374

10.1677/JOE-08-0395

http://dx.doi.org/10.1677/JOE-08-0395

Idioma(s)

eng

Publicador

BIOSCIENTIFICA LTD

Relação

Journal of Endocrinology

Direitos

restrictedAccess

Copyright BIOSCIENTIFICA LTD

Palavras-Chave #GASTRIC-INHIBITORY POLYPEPTIDE #MACRONODULAR ADRENAL-HYPERPLASIA #LUTEINIZING-HORMONE RECEPTOR #CUSHINGS-SYNDROME SECONDARY #GROWTH-FACTOR-II #ABERRANT EXPRESSION #ECTOPIC EXPRESSION #GIP RECEPTOR #MOLECULAR PATHOGENESIS #CORTICAL CARCINOMA #Endocrinology & Metabolism
Tipo

article

original article

publishedVersion