HER2 Testing in Breast Carcinoma Very Low Concordance Rate Between Reference and Local Laboratories in Brazil


Autoria(s): WLUDARSKI, Sheila Cristina Lordelo; LOPES, Lisandro Ferreira; SILVA, Tacio R. Berto e; CARVALHO, Filomena M.; WEISS, Lawrence M.; BACCHI, Carlos E.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

Breast cancer accounts for approximately one quarter of all cancers in females. HER2 gene amplification or HER2 protein overexpression, detected in about 20% of breast carcinomas, predicts a more aggressive clinical course and determines eligibility for targeted therapy with trastuzumab. HER2 testing has become an essential part of the clinical evaluation of all breast carcinoma patients, and accurate HER2 results are critical in identifying patients who may be benefited from targeted therapy. This study investigated the concordance in the results of HER2 immunohistochemistry assays performed in 500 invasive breast carcinomas between a reference laboratory and 149 local laboratories from all geographic regions of Brazil. Our results showed an overall poor concordance (171 of 500 cases, 34.2%) regarding HER2 results between local and reference laboratories, which may be related to the low-volume load of HER2 assays, inexperience with HER2 scoring system, and/or technical issues related to immunohistochemistry in local laboratories. Standardization of HER2 testing with rigorous quality control measures by local laboratories is highly recommended to avoid erroneous treatment of breast cancer patients.

Identificador

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, v.19, n.2, p.112-118, 2011

1062-3345

http://producao.usp.br/handle/BDPI/22852

10.1097/PAI.0b013e3181f0b044

http://dx.doi.org/10.1097/PAI.0b013e3181f0b044

Idioma(s)

eng

Publicador

LIPPINCOTT WILLIAMS & WILKINS

Relação

Applied Immunohistochemistry & Molecular Morphology

Direitos

restrictedAccess

Copyright LIPPINCOTT WILLIAMS & WILKINS

Palavras-Chave #Brazil #HER2 #immunohistochemistry #local laboratory #reference laboratory #HER-2/NEU GENE AMPLIFICATION #RABBIT MONOCLONAL-ANTIBODY #SITU HYBRIDIZATION FISH #OF-AMERICAN-PATHOLOGISTS #ARCHIVAL TISSUE SAMPLES #CANCER #IMMUNOHISTOCHEMISTRY #TRASTUZUMAB #ONCOGENE #IHC #Anatomy & Morphology #Medical Laboratory Technology #Pathology
Tipo

article

original article

publishedVersion