Effects of bone marrow-derived mononuclear cells on airway and lung parenchyma remodeling in a murine model of chronic allergic inflammation


Autoria(s): ABREU, Soraia C.; ANTUNES, Mariana A.; MARON-GUTIERREZ, Tatiana; CRUZ, Fernanda F.; CARMO, Luana G. R. R.; ORNELLAS, Debora S.; CARREIRA JUNIOR, Humberto; ABISABER, Alexandre M.; PARRA, Edwin R.; CAPELOZZI, Vera L.; MORALES, Marcelo M.; ROCCO, Patricia R. M.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

We hypothesized that bone marrow-derived mononuclear cells (BMDMC) would attenuate the remodeling process in a chronic allergic inflammation model. C57BL/6 mice were assigned to two groups. In OVA, mice were sensitized and repeatedly challenged with ovalbumin. Control mice (C) received saline under the same protocol. C and OVA were further randomized to receive BMDMC (2 x 10(6)) or saline intravenously 24 h before the first challenge. BMDMC therapy reduced eosinophil infiltration, smooth muscle-specific actin expression, subepithelial fibrosis, and myocyte hypertrophy and hyperplasia, thus causing a decrease in airway hyperresponsiveness and lung mechanical parameters. BMDMC from green fluorescent protein (GFP)-transgenic mice transplanted into GFP-negative mice yielded lower engraftment in OVA. BMDMC increased insulin-like growth factor expression, but reduced interleukin-5, transforming growth factor-beta, platelet-derived growth factor, and vascular endothelial growth factor mRNA expression. In conclusion, in the present chronic allergic inflammation model, BMDMC therapy was an effective pre-treatment protocol that potentiated airway epithelial cell repair and prevented inflammatory and remodeling processes. (C) 2010 Elsevier B.V. All rights reserved.

Centers of Excellence Program (PRONEX-FAPERJ)

Brazilian Council for Scientific and Technological Development (CNPq)

Rio de Janeiro State Research Supporting Foundation (FAPERJ)

Coordination for the Improvement of Higher Education Personnel (CAPES)

Sao Paulo State Research Supporting Foundation (FAPESP)

Identificador

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, v.175, n.1, p.153-163, 2011

1569-9048

http://producao.usp.br/handle/BDPI/22741

10.1016/j.resp.2010.10.006

http://dx.doi.org/10.1016/j.resp.2010.10.006

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Respiratory Physiology & Neurobiology

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Inflammation #Collagen fiber #Lung mechanics #Stem cells #Asthma #MESENCHYMAL STEM-CELLS #INJURY #REPAIR #ENGRAFTMENT #MICE #PLASTICITY #PULMONARY #THERAPY #ASTHMA #Physiology #Respiratory System
Tipo

article

original article

publishedVersion