Low dose of fine particulate matter (PM2.5) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
19/10/2012
19/10/2012
2011
|
Resumo |
Air pollution is associated with morbidity and mortality induced by respiratory diseases. However, the mechanisms therein involved are not yet fully clarified. Thus, we tested the hypothesis that a single acute exposure to low doses of fine particulate matter (PM2.5) may induce functional and histological lung changes and unchain inflammatory and oxidative stress processes. PM2.5 was collected from the urban area of Sao Paulo city during 24 h and underwent analysis for elements and polycyclic aromatic hydrocarbon contents. Forty-six male BALB/c mice received intranasal instillation of 30 mu L of saline (CTRL) or PM2.5 at 5 or 15 mu g in 30 mu L of saline (P5 and P15, respectively). Twenty-four hours later, lung mechanics were determined. Lungs were then prepared for histological and biochemical analysis. P15 group showed significantly increased lung impedance and alveolar collapse, as well as lung tissue inflammation, oxidative stress and damage. P5 presented values between CTRL and P15: higher mechanical impedance and inflammation than CTRL, but lower inflammation and oxidative stress than P15. In conclusion, acute exposure to low doses of fine PM induced lung inflammation, oxidative stress and worsened lung impedance and histology in a dose-dependent pattern in mice. The Centers of Excellence Program (PRONEX-MCT/FAPERJ) The Brazilian Council for Scientific and Technological Development (MCT/CNPq) The Carlos Chagas Filho Rio de Janeiro State Research Supporting Foundation (FAPERJ) |
Identificador |
INHALATION TOXICOLOGY, v.23, n.5, p.257-267, 2011 0895-8378 http://producao.usp.br/handle/BDPI/22732 10.3109/08958378.2011.566290 |
Idioma(s) |
eng |
Publicador |
INFORMA HEALTHCARE |
Relação |
Inhalation Toxicology |
Direitos |
restrictedAccess Copyright INFORMA HEALTHCARE |
Palavras-Chave | #Particulate matter #PM2.5 #lung injury #oxidative stress #respiratory mechanics #DIESEL EXHAUST PARTICLES #POLYCYCLIC AROMATIC-HYDROCARBONS #ALVEOLAR PRESSURE MEASUREMENT #AIR-POLLUTION PARTICLES #TRANSITION-METALS #LUNG INJURY #IN-VITRO #FLY-ASH #EPITHELIAL-CELLS #POLYMORPHONUCLEAR LEUKOCYTES #Toxicology |
Tipo |
article original article publishedVersion |