Immunophenotyping and remodeling process in small airways of idiopathic interstitial pneumonias: functional and prognostic significance


Autoria(s): PARRA, E. R.; NOLETO, G. S.; TINOCO, L. J. M.; CAPELOZZI, V. L.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background and Aims: To test whether different degrees of immunologic and fibrotic airway remodeling processes occur in idiopathic interstitial pneumonias (IIPs), with impact on functional tests and survival, we studied the collagen/elastic system and immune cell density in the bronchiolar interstitium of lungs with the major types of IIPs. Materials and Methods: Histochemistry, immunohistochemistry and morphometric analysis were used to evaluate collagen/elastic fibers and immune cells in the bronchiolar interstitium of open lung biopsies of patients with cryptogenic organizing pneumonia [COP/organizing pneumonia (OP) = 10], acute interstitial pneumonia [AIP/diffuse alveolar damage (DAD) = 20], nonspecific interstitial pneumonia (NSIP/NSIP = 20) and idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP) = 20. Results: OP lungs presented a significant increase in collagenous/elastic fibers and in the total density of immune cells in the bronchiolar interstitium compared to controls, DAD, NSIP and UIP. We observed a significant increase in CD4, CD8 and CD20 lymphocytes, as well as in neutrophils, macrophages and plasma cells in OP. The increased amount of elastic fibers in the bronchiolar interstitium from OP lungs has a direct association with forced vital capacity (FVC) (r(s) = 0.99, P = 0.03). The most important survival predictor was CD20+ lymphocytes in the bronchiolar interstitium. In decreasing order, patients with UIP [Odds Ratio (OR) = 35.01], high forced expiratory volume in 1 s (FEV1)/FVC FVC (OR = 7.01), increased CD20+ lymphocytes (OR = 4.44) and collagenous/elastic fiber densities (OR = 2.03 and OR = 1.49, respectively) in the bronchiolar interstitium were those who had the greatest risk of death, followed by those with AIP, NSIP and COP. Conclusion: Different degrees of immunologic and fibroelastotic airway remodeling processes occur in the major types of IIPs with impact on physiological tests and survival.

Brazilian agencies

National Council for Scientific and Technological Development

FAPESP Foundation for the Support of Research of the State of Sao Paulo[06/54978-8]

FAPESP Foundation for the Support of Research of the State of Sao Paulo[06/51260-9]

FAPESP Foundation for the Support of Research of the State of Sao Paulo[06/51400-5]

Universidade de São Paulo - Laboratories for Medical Research[LIM 05] - FM/USP

Clinicas Hospital

School of Medicine, University of Sao Paulo (USP)

Identificador

CLINICAL RESPIRATORY JOURNAL, v.2, n.4, p.227-238, 2008

1752-6981

http://producao.usp.br/handle/BDPI/22673

10.1111/j.1752-699X.2008.00077.x

http://dx.doi.org/10.1111/j.1752-699X.2008.00077.x

Idioma(s)

eng

Publicador

BLACKWELL PUBLISHING

Relação

Clinical Respiratory Journal

Direitos

closedAccess

Copyright BLACKWELL PUBLISHING

Palavras-Chave #airway #collagen #elastic system #functional testing #idiopathic interstitial pneumonias #immune cells #survival #LUNG-DISEASE #FIBROSING ALVEOLITIS #ORGANIZING PNEUMONIA #PULMONARY-FIBROSIS #BRONCHIOLITIS #COLLAGEN #FIBERS #Respiratory System
Tipo

article

original article

publishedVersion