Quantitative ferromagnetic resonance analysis of CD133 stem cells labeled with iron oxide nanoparticles


Autoria(s): GAMARRA, L. F.; PAVON, L. F.; MARTI, L. C.; PONTUSCHKA, W. M.; MAMANI, J. B.; COSTA-FILHO, A. J.; VIEIRA, E. D.; MOREIRA-FILHO, C. A.; AMARO JR., E.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

The aim of this work is to provide a quantitative method for analysis of the concentration of superparamagnetic iron oxide nanoparticles (SPION), determined by means of ferromagnetic resonance (FMR), with the nanoparticles coupled to a specific antibody (AC133), and thus to express the antigenic labeling evidence for the stem cells C D133(+). The FMR efficiency and sensitivity were proven adequate for detecting and quantifying the low amounts of iron content in the C D133(+) cells (similar to 6.16 x 10(5) pg in the volume of 2 mu l containing 4.5 x 1011 SPION). The quantitative method led to the result of 1.70 x 10(-13) mol of Fe (9.5 pg), or 7.0 x 10(6) nanoparticles per cell. For the quantification analysis via the FMR technique it was necessary to carry out a preliminary quantitative visualization of iron oxide-labeled cells in order to ensure that the nanoparticles coupled to the antibodies are indeed tied to the antigen at the stem cell surface and that the cellular morphology was conserved, as proof of the validity of this method. The quantitative analysis by means of FMR is necessary for determining the signal intensity for the study of molecular imaging by means of magnetic resonance imaging (MRI).

Identificador

JOURNAL OF PHYSICS-CONDENSED MATTER, v.20, n.20, 2008

0953-8984

http://producao.usp.br/handle/BDPI/21971

10.1088/0953-8984/20/20/204150

http://dx.doi.org/10.1088/0953-8984/20/20/204150

Idioma(s)

eng

Publicador

IOP PUBLISHING LTD

Relação

Journal of Physics-condensed Matter

Direitos

restrictedAccess

Copyright IOP PUBLISHING LTD

Palavras-Chave #CONTRAST AGENTS #BONE-MARROW #TRACKING #FERROFLUIDS #ANTIGEN #SCANNER #Physics, Condensed Matter
Tipo

article

proceedings paper

publishedVersion