PCR-mediated detection of acidophilic, bioleaching-associated bacteria


Autoria(s): DeWulfDurand, P; Bryant, LJ; Sly, LI
Data(s)

01/01/1997

Resumo

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

Identificador

http://espace.library.uq.edu.au/view/UQ:57771/UQ57771_OA.pdf

http://espace.library.uq.edu.au/view/UQ:57771

Idioma(s)

eng

Palavras-Chave #Biotechnology & Applied Microbiology #Microbiology #Thiobacillus-ferrooxidans #Leptospirillum-ferrooxidans #Solid Medium #Rapid Method #Environments #Sulfur #Enumeration
Tipo

Journal Article