Development of an animal model for allergic conjunctivitis: influence of genetic factors and allergen concentration on immune response
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2008
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Resumo |
Purpose: Animal models of diseases are extremely important in the study of the physiopathogenesis of human diseases and for testing novel therapeutic interventions. The present study aimed to develop an animal model that simulates human allergic conjunctivitis and to study how allergic response may be influenced by the allergen dose used for immunization and by genetic factors. Methods: Sixty C57Bl/6 mice and 60 BALB/c mice were immunized with placebo, or 5 mu g or 500 mu g of allergen derived from Dermatophagoides pteronyssinus. After ocular challenge, the mice were examined in order to clinically verify the occurrence or not of conjunctivitis. Material obtained from animals was used for total and specific IgE and IgG1 dosage, for assays of Der p-specific lymphocyte proliferation and supernatant cytokine dosage, and for histopathological evaluation of conjunctiva. Results: We developed a murine model of allergic conjunctivitis induced by D. pteronyssinus. The model is similar to human disease both clinically and according to laboratory findings. In mouse, conjunctivitis was associated with a Th2 cytokine profile. However, IL-10 appeared to be involved with disease blockade. Mice of different strains have distinct immune responses, depending on the sensitization dose. Conclusions: The murine model developed is suitable for the study of immunopathogenesis and as a template for future therapies. Using BALB/c and C57BL/6 mice, we demonstrated that genetic factors play a role in determining susceptibility and resistance, as well as in establishing the allergen concentration needed to induce or to block disease development. |
Identificador |
ACTA OPHTHALMOLOGICA, v.86, n.6, p.670-675, 2008 1755-375X http://producao.usp.br/handle/BDPI/21634 10.1111/j.1600-0420.2007.01134.x |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL |
Relação |
Acta Ophthalmologica |
Direitos |
restrictedAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #animal models of diseases #allergic conjunctivitis #immediate hypersensitivity #mice #mucosal immunity #mites #T-CELL-CLONES #MURINE MODEL #EYE DISEASE #MOUSE MODEL #RHINOCONJUNCTIVITIS #NUMBERS #ANTIGEN #ASTHMA #IL-10 #GAMMA #Ophthalmology |
Tipo |
article original article publishedVersion |