In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
---|---|
Data(s) |
19/10/2012
19/10/2012
2011
|
Resumo |
Aims: To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squanous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT). Methods and results: Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells. Conclusion: HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features. FAPESP[01/13644-6] CAPES CNPq |
Identificador |
HISTOPATHOLOGY, v.58, n.2, p.225-233, 2011 0309-0167 http://producao.usp.br/handle/BDPI/21494 10.1111/j.1365-2559.2011.03751.x |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL PUBLISHING, INC |
Relação |
Histopathology |
Direitos |
restrictedAccess Copyright WILEY-BLACKWELL PUBLISHING, INC |
Palavras-Chave | #homeobox genes #oral squamous cell carcinoma #HOX GENES #CLINICOPATHOLOGICAL SIGNIFICANCE #THYROID-CANCER #HEAD #NECK #HOMEODOMAIN #PITX1 #DISEASE #ORGANS #PTX1 #Cell Biology #Pathology |
Tipo |
article original article publishedVersion |