In situ hybridization detection of homeobox genes reveals distinct expression patterns in oral squamous cell carcinomas


Autoria(s): LIBORIO, Tatiana N.; ACQUAFREDA, Thais; MATIZONKAS-ANTONIO, Luciana F.; SILVA-VALENZUELA, Maria G.; FERRAZ, Alberto R.; NUNES, Fabio D.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

Aims: To analyse the expression of three homeobox genes (HOXA7, PITX1 and PRRX1) in oral squanous cell carcinomas (OSCC) and the relationship of such expression to certain distinct histopathological features of OSCC and in comparison to adjacent non-neoplastic epithelium (NT). Methods and results: Digoxigenin-labelled riboprobes that are specific for each homeobox gene were generated and in situ hybridization was carried out on frozen sections. In NT samples, HOXA7 and PITX1 transcripts were found more frequently in all epithelial layers, while PRRX1 was expressed in the basal layer. With OSCC samples, expression of the three genes was associated with all histological features. However, the HOXA7 and PITX1 signals were more intense in sheets and nests and PRRX1 in small nests and isolated cells. Conclusion: HOXA7, PIXT1 and PRRX1 homeobox genes have different patterns of expression in OSCC depending on its histological features.

FAPESP[01/13644-6]

CAPES

CNPq

Identificador

HISTOPATHOLOGY, v.58, n.2, p.225-233, 2011

0309-0167

http://producao.usp.br/handle/BDPI/21494

10.1111/j.1365-2559.2011.03751.x

http://dx.doi.org/10.1111/j.1365-2559.2011.03751.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL PUBLISHING, INC

Relação

Histopathology

Direitos

restrictedAccess

Copyright WILEY-BLACKWELL PUBLISHING, INC

Palavras-Chave #homeobox genes #oral squamous cell carcinoma #HOX GENES #CLINICOPATHOLOGICAL SIGNIFICANCE #THYROID-CANCER #HEAD #NECK #HOMEODOMAIN #PITX1 #DISEASE #ORGANS #PTX1 #Cell Biology #Pathology
Tipo

article

original article

publishedVersion