Gut ischemia/reperfusion induced acute lung injury is an alveolar macrophage dependent event


Autoria(s): MORAES, Luciana Borsoi; MURAKAINI, Abel Hiroshi F.; FONTES, Belchor; POGGETTI, Renato Sergio; ROOIJEN, Nico van; YOUNES, Riad Nain; HEIMBECKER, Ana Maria Cattani; BIROLINI, Dario
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background:. Although the role of the lung alveolar macrophage (AM) as a mediator of acute lung injury (ALI) after lung ischemia/reperfusion (I/R) has been suggested by animal experiments, it has not been determined whether AMs mediate ALI after intestinal I/R. The objective of this study was to determine the effect of AM elimination on ALI after intestinal I/R in rats. Mwthods: Male Wistar rats (n = 90) were randomly divided into three groups: the clodronate-liposomes (CLOD-LIP) group received intratracheal treatment with CLOD-LIP; the liposomes (LIP) group received intratracheal treatment with LIP; and the nontreated (UNTREAT) group received no treatment. Twenty-four hours later each group was randomly divided into three subgroups: the intestinal I/R subgroup was subjected to 45-minute intestinal ischemia and 2-hour reperfusion; the laparotomy (LAP) subgroup was subjected to LAP and sham procedures; the control (CTR) subgroup received no treatment. At the end of reperfusion, ALI was quantitated in all the animals by the Evans blue dye (EBD) method. Results: ALI values are expressed as EBD lung leakage (mu g EBD/g dry lung weight). EBD lung leakage values in the CLOD-LIP group were 32.59 +/- 12.74 for I/R, 27.74 +/- 7.99 for LAP, and 33.52 +/- 10.17 for CTR. In the LIP group, lung leakage values were 58.02 +/- 18.04 for I/R, 31.90 +/- 8.72 for LAP, and 27.17 +/- 11.48 for CTR. In the UNTREAT group, lung leakage values were 55.60 +/- 10.96 for I/R, 35.99 +/- 6.89 for LAP, and 30.83 +/- 8.41 for CTR. Within each group, LAP values did not differ from CTR values. However, in the LIP and UNTREAT groups, values for both the LAP and CTR subgroups were lower than values for the I/R subgroup (p < 0.001). The CLOD-LIP I/R subgroup value was less (p < 0.001) than the I/R subgroup values in the LIP and UNTREAT groups. These results indicated that I/R provokes ALI that can be prevented by CLOD-LIP treatment, and further suggested that AMs are essential for ALI occurrence induced by intestinal I/R in rats.

Identificador

JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, v.64, n.5, p.1196-1200, 2008

0022-5282

http://producao.usp.br/handle/BDPI/21463

10.1097/TA.0b013e31816c5ca6

http://dx.doi.org/10.1097/TA.0b013e31816c5ca6

Idioma(s)

eng

Publicador

LIPPINCOTT WILLIAMS & WILKINS

Relação

Journal of Trauma-injury Infection and Critical Care

Direitos

restrictedAccess

Copyright LIPPINCOTT WILLIAMS & WILKINS

Palavras-Chave #clodronate-liposomes #acute lung injury #alveolar macrophage #intestinal ischemia #ISCHEMIA-REPERFUSION INJURY #ENCAPSULATED DICHLOROMETHYLENE DIPHOSPHONATE #TUMOR-NECROSIS-FACTOR #DEPLETION #ORGAN #ELIMINATION #MICE #ACTIVATION #CLODRONATE #LIPOSOMES #Critical Care Medicine #Surgery
Tipo

article

proceedings paper

publishedVersion