Mannan-binding lectin MBL2 gene polymorphism in chronic hepatitis C: association with the severity of liver fibrosis and response to interferon therapy


Autoria(s): PEDROSO, M. L. Alves; BOLDT, A. B. W.; PEREIRA-FERRARI, L.; STEFFENSEN, R.; STRAUSS, E.; JENSENIUS, J. C.; IOSHII, S. O.; MESSIAS-REASON, I.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Hepatitis C virus (HCV) is a major cause of hepatic disease and of liver transplantation worldwide. Mannan-binding lectin (MBL), encoded by the MBL2 gene, can have an important role as an opsonin and complement activating molecule in HCV persistence and liver injury. We assessed the MBL2 polymorphism in 102 Euro-Brazilian patients with moderate and severe chronic hepatitis C, paired for gender and age with 102 HCV seronegative healthy individuals. Six common single nucleotide polymorphisms in the MBL2 gene, three in the promoter (H/L, X/Y and P/Q) and three in exon 1 (A, the wild-type, and B, C or D also known as O) were evaluated using real-time polymerase chain reaction with fluorescent hybridization probes. The concentration of MBL in plasma was measured by enzyme-linked immunosorbent assay. The frequency of the YA/YO genotype was significantly higher in the HCV patients compared with the controls (P = 0.022). On the other hand, the genotypes associated with low levels of MBL (XA/XA, XA/YO and YO/YO) were decreased significantly in the patients with severe fibrosis (stage F4), when compared with the patients with moderate fibrosis (stage F2) (P = 0.04) and to the control group (P = 0.011). Furthermore, MBL2 genotypes containing X or O mutations were found to be associated with non-responsiveness to pginterferon and ribavirin treatment (P = 0.023). MBL2 polymorphisms may therefore be associated not only with the development of chronic hepatitis C, but also with its clinical evolution and response to treatment.

Identificador

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, v.152, n.2, p.258-264, 2008

0009-9104

http://producao.usp.br/handle/BDPI/21353

10.1111/j.1365-2249.2008.03614.x

http://dx.doi.org/10.1111/j.1365-2249.2008.03614.x

Idioma(s)

eng

Publicador

BLACKWELL PUBLISHING

Relação

Clinical and Experimental Immunology

Direitos

restrictedAccess

Copyright BLACKWELL PUBLISHING

Palavras-Chave #HCV #hepatitis C #liver fibrosis #mannan-binding lectin #MBL #VIRUS-INFECTION #MUTATIONS #DEFICIENCY #PROTEIN #SUSCEPTIBILITY #PROMOTER #ALLELES #DISEASE #EPIDEMIOLOGY #POPULATIONS #Immunology
Tipo

article

original article

publishedVersion