Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer


Autoria(s): CASTILLO, Hozana A.; CRAVO, Roberta M.; AZAMBUJA, Ana P.; SIMOES-COSTA, Marcos S.; SURA-TRUEBA, Sylvia; GONZALEZ, Jose; SLONIMSKY, Esfir; ALMEIDA, Karla; ABREU, Jose G.; ALMEIDA, Marcio A. Afonso de; SOBREIRA, Tiago P.; OLIVEIRA, Saulo H. Pires de; OLIVEIRA, Paulo S. Lopes de; SIGNORE, Iskra A.; COLOMBO, Alicia; CONCHA, Miguel L.; SPENGLER, Tatjana S.; BRONNER-FRASER, Marianne; NOBREGA, Marcelo; ROSENTHAL, Nadia; XAVIER-NETO, Jose
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dl1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dl1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception.

FAPESP[02/11340-2]

FAPESP[04/11569-5]

FAPESP[04/15704-4]

FAPESP[05/60637-6]

FAPESP[06/50843-0]

FAPESP[06/61317-8]

CNPq[305260/2007-3]

Company of Biologists

Identificador

DEVELOPMENT, v.137, n.3, p.507-518, 2010

0950-1991

http://producao.usp.br/handle/BDPI/21189

10.1242/dev.043257

http://dx.doi.org/10.1242/dev.043257

Idioma(s)

eng

Publicador

COMPANY OF BIOLOGISTS LTD

Relação

Development

Direitos

restrictedAccess

Copyright COMPANY OF BIOLOGISTS LTD

Palavras-Chave #Retinoic acid #Spinal cord #Roof plate #Commissural interneurons #Proprioception #Paired fin loss #Mouse #Chicken #Xenopus #Zebrafish #Lamprey #Medaka #RETINOIC ACID SYNTHESIS #DEHYDROGENASE TYPE-2 #NEURAL CREST #EXPRESSION #GENE #ORIGIN #MOUSE #ZEBRAFISH #SPECIFICATION #VERTEBRATES #Developmental Biology
Tipo

article

original article

publishedVersion