The role of CD4 T help in multiple vaccinations when using minimal CD8 T cell epitope peptides


Autoria(s): Khammanivong, V.; Peters, T.; Andrews, M.; Leggatt, G. R.; Frazer, I. H.; Fernando, G. J.
Data(s)

01/11/2005

Resumo

Background: The fact that some cancers and viral infections can be controlled by effector CD8 T cells led to the possibility of utilising minimal CD8 T cell epitope peptides as vaccines. However using minimal CD8 T cell epitope peptide immunisations and a tumour protection model in mice, we have previously shown that functional memory CD8 T cells are not generated unless CD4 T help is provided at the time of CD8 T cell priming. Short-lived effector cells nevertheless are generated in the absence of T help. Aim: To determine the role of CD4 T help in multiple immunisations. Method: Minimal CD8 T cell peptides of HPV16 E7 protein and Ovalbumin were used (with adjuvants Quil-A or IFA) as immunogens in C57BL mice. The presence of effector CD8 T cells were determined by tumour protection assays and was quantified by IFN-gamma ELISPOT assays. Results: In the present study we show that unless T help is provided at the time CD8 T cells are primed, no CD8 effector cells are generated when boosted with the vaccine again in the absence of T help. Our results further show that this failure could be prevented by the inclusion of a T helper peptide during the primary or booster immunisations.

Identificador

http://espace.library.uq.edu.au/view/UQ:56048

Idioma(s)

eng

Publicador

Blackwell Publishing

Palavras-Chave #Cell Biology #Immunology #Pathology
Tipo

Journal Article