Social separation and diazepam withdrawal increase anxiety in the elevated plus-maze and serotonin turnover in the median raphe and hippocampus


Autoria(s): SANTOS, Lucineia dos; ANDRADE, Telma G. C. S. de; GRAEFF, Frederico G.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

The present work aimed to evaluate the effects of social separation for 14 days (chronic stress) and of withdrawal from a 14-day treatment with diazepam (acute stress) on the exploratory behaviour of male rats in the elevated plus-maze and on serotonin (5-hydroxytryptamine) turnover in different brain structures. Social separation had an anxiogenic effect, evidenced by fewer entries into, and less time spent on the open arms of the elevated plus-maze. Separation also selectively increased 5-hydroxytryptamine turnover in the hippocampus and median raphe nucleus. Diazepam withdrawal had a similar anxiogenic effect in grouped animals and increased 5-hydroxytryptamine turnover in the same brain structures. Chronic treatment with imipramine during the 14 days of separation prevented the behavioural and neurochemical changes caused by social separation. It is suggested that the increase in anxiety determined by both acute and chronic stress is mediated by the activation of the median raphe nucleus-hippocampal 5-hydroxytryptamine pathway.

State of Sao Paulo Research Foundation (FAPESP)

National Council for Scientific and Technological Development (CNPq)

Foundation of the Support to Teaching, Research and Assistance, Clinical Hospital, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo (FAEPA/USP)

Identificador

JOURNAL OF PSYCHOPHARMACOLOGY, v.24, n.5, p.725-731, 2010

0269-8811

http://producao.usp.br/handle/BDPI/20888

10.1177/0269881109106954

http://dx.doi.org/10.1177/0269881109106954

Idioma(s)

eng

Publicador

SAGE PUBLICATIONS LTD

Relação

Journal of Psychopharmacology

Direitos

restrictedAccess

Copyright SAGE PUBLICATIONS LTD

Palavras-Chave #social separation #anxiety #elevated plus-maze #serotonin #diazepam withdrawal #imipramine #T-MAZE #5-HT1A RECEPTORS #CHRONIC IMIPRAMINE #BENZODIAZEPINE WITHDRAWAL #DORSAL HIPPOCAMPUS #NUCLEUS #RAT #BEHAVIOR #STRESS #ACTIVATION #Clinical Neurology #Neurosciences #Pharmacology & Pharmacy #Psychiatry
Tipo

article

original article

publishedVersion