Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection


Autoria(s): CHAVES, Andrea R.; LEANDRO, Fernanda Z.; CARRIS, Juciene A.; QUEIROZ, Maria Eugenia C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

A sensitive and reproducible method by microextraction packed sorbent and liquid chromatography with UV detection (MEPS/LC-UV) is described for the determination of new generation antidepressants (sertraline, mirtazapine, fluoxetine, citalopram and paroxetine) in human plasma samples. The MEPS variables, such as sample volume, pH, number of extraction cycles (draw-eject), and desorption conditions (solvent and solvent volume of elution) influenced the MEPS/LC efficiency significantly. Important factors in the optimization of MEPS efficiency, as well as washing steps and carryover effect are discussed. The analyses were carried out using small sample volumes (400 mu L.), and in a short time period (3 min for the entire sample preparation step). The MEPS/LC-UV method was shown to be linear at concentrations ranging from the limit of quantification (LOQ) to 1000 ng mL(-1). The LOQ values ranged from 10 to 25 ng mL(-1). The inter-day precision of the method presented coefficient of the variation ranging from 1.3% to 8.7%. On the basis of analytical validation, it is shown that the MEPS/LC-UV methodology is adequate for antidepressant analysis, from therapeutic to toxic levels. In order to evaluate the proposed method for clinical use, the MEPS/LC-UV method was applied to analysis of plasma samples from elderly depressed patients. (C) 2010 Elsevier B.V. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Identificador

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.878, n.23, p.2123-2129, 2010

1570-0232

http://producao.usp.br/handle/BDPI/20820

10.1016/j.jchromb.2010.06.023

http://dx.doi.org/10.1016/j.jchromb.2010.06.023

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Microextraction in packed sorbent #Sample preparation #Antidepressants #Liquid chromatography #Human plasma samples #TANDEM MASS-SPECTROMETRY #SAMPLES UTILIZING MICROEXTRACTION #MEPS-LC-MS/MS #URINE SAMPLES #SYRINGE MEPS #ONLINE #QUANTIFICATION #EXTRACTION #ROPIVACAINE #PERFORMANCE #Biochemical Research Methods #Chemistry, Analytical
Tipo

article

original article

publishedVersion