Solid-phase microextraction using poly(pyrrole) film and liquid chromatography with UV detection for analysis of antidepressants in plasma samples


Autoria(s): CHAVES, Andrea Rodrigues; CHIERICATO JUNIOR, Glaico; QUEIROZ, Maria Eugema Costa
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

Poly(pyrrole) (PPY) coating was prepared on a stainless-steel (SS) wire for solid-phase microextraction (SPME) by electrochemical deposition (cyclic voltammetric). The PPY was evaluated by analyzing new-generation antidepressants (mirtazapine, citalopram, paroxetine, duloxetine, fluoxetine, and sertraline) in plasma sample by SPME and liquid chromatography with UV detection (LC-UV). The effect of electrolyte Solution (lithium perchlorate or tetrabutylammonium perchlorate) and the number of cycles (50, 100 or 200) applied during the polymerization process on the SPME performance was evaluated. Important factors in the optimization of SPME efficiency such as extraction time, temperature, pH, influence of plasma proteins on sorption mechanisms, and desorption conditions are discussed. The SPME-PPY/LC method showed to be linear in concentrations ranging from the limit of quantification (LOQ) to 1200 ng mL(-1). The LOQ values range from 16 to 25 ng mL-1. The inter-day precision of the SPME-PPY/LC method presented coefficient of variation (CV) lower than 15%. Based on analytical validation results, the SPME-PPY/LC methodology showed to be adequate for antidepressant analysis, from therapeutic to toxic levels. In order to evaluate the proposed method for clinical use, the SPME-PPY/LC method was applied to the analysis of plasma samples from elderly depressed patients. (c) 2009 Elsevier B.V. All rights reserved,

Funcao de Amparo A Pesquisa do Estado cle Sao Paulo (FAPESP)

Conselho Nacional cle Desenvolvimento Cientifico e Tecriologico (CNPq)

Identificador

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.877, n.7, p.587-593, 2009

1570-0232

http://producao.usp.br/handle/BDPI/20709

10.1016/j.jchromb.2008.12.070

http://dx.doi.org/10.1016/j.jchromb.2008.12.070

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Antidepressants #Poly(pyrrole) #SPME #Liquid chromatography #TRICYCLIC ANTIDEPRESSANTS #NONTRICYCLIC ANTIDEPRESSANTS #GAS-CHROMATOGRAPHY #MASS-SPECTROMETRY #POLYPYRROLE FILMS #ACTIVE METABOLITES #HPLC METHOD #EXTRACTION #SPME #SERUM #Biochemical Research Methods #Chemistry, Analytical
Tipo

article

original article

publishedVersion