Production of xylanase by Aspergilli using alternative carbon sources: application of the crude extract on cellulose pulp biobleaching


Autoria(s): PEIXOTO-NOGUEIRA, Simone de Carvalho; MICHELIN, Michele; BETINI, Jorge Henrique Almeida; JORGE, Joao Atilio; TERENZI, Hector Francisco; POLIZELI, Maria de Lourdes Teixeira de Moraes
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

The ability of xylanolytic enzymes produced by Aspergillus fumigatus RP04 and Aspergillus niveus RP05 to promote the biobleaching of cellulose pulp was investigated. Both fungi grew for 4-5 days in liquid medium at 40A degrees C, under static conditions. Xylanase production was tested using different carbon sources, including some types of xylans. A. fumigatus produced high levels of xylanase on agricultural residues (corncob or wheat bran), whereas A. niveus produced more xylanase on birchwood xylan. The optimum temperature of the xylanases from A. fumigatus and A. niveus was around 60-70A degrees C. The enzymes were stable for 30 min at 60A degrees C, maintaining 95-98% of the initial activity. After 1 h at this temperature, the xylanase from A. niveus still retained 85% of initial activity, while the xylanase from A. fumigatus was only 40% active. The pH optimum of the xylanases was acidic (4.5-5.5). The pH stability for the xylanase from A. fumigatus was higher at pH 6.0-8.0, while the enzyme from A. niveus was more stable at pH 4.5-6.5. Crude enzymatic extracts were used to clarify cellulose pulp and the best result was obtained with the A. niveus preparation, showing kappa efficiency around 39.6% as compared to only 11.7% for that of A. fumigatus.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientfico e Tecnologico (CNPq)

Identificador

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.36, n.1, p.149-155, 2009

1367-5435

http://producao.usp.br/handle/BDPI/20688

10.1007/s10295-008-0482-y

http://dx.doi.org/10.1007/s10295-008-0482-y

Idioma(s)

eng

Publicador

SPRINGER HEIDELBERG

Relação

Journal of Industrial Microbiology & Biotechnology

Direitos

restrictedAccess

Copyright SPRINGER HEIDELBERG

Palavras-Chave #Aspergillus #Xylanase #Biobleaching #Cellulose pulp #Filamentous fungi #KRAFT PULP #INDUSTRIAL APPLICATIONS #THERMOTOLERANT FUNGUS #HUMICOLA-GRISEA #WHEAT-STRAW #PURIFICATION #TEMPERATURE #PHOENICIS #FUMIGATUS #ENZYMES #Biotechnology & Applied Microbiology
Tipo

article

original article

publishedVersion