Quantification of free mycophenolic acid by high-performance liquid chromatography-atmospheric pressure chemical ionisation tandem mass spectrometry


Autoria(s): Willis, C.; Taylor, P. J.; Salm, P.; Tett, S. E.; Pillans, P. I.
Data(s)

01/10/2000

Resumo

To facilitate the investigation of free mycophenolic acid concentrations we developed a high-performance liquid chromatography tandem mass spectrometry method using indomethacin as an internal standard. Free drug was isolated from plasma samples (500 mul) using ultrafiltration, The analytes were extracted from the ultrafiltrate (200 mul) using C-18 solid-phase extraction. Detection was by selected reactant monitoring of mycophenolic acid (m/z 318.9-->190.9) and the internal standard (m/z 356.0-->297.1) with an atmospheric pressure chemical ionisation interface. The total chromatographic analysis time was 12 min. The method was found to be linear over the range investigated, 2.5-200 mug/l (r>0.990, n=6). The relative recovery of the method for the control samples studied (7.5, 40.0 and 150 mug/l) ranged from 95 to 104%. The imprecision of the method, expressed in terms of intra- and inter-day coefficients of variation, was

Identificador

http://espace.library.uq.edu.au/view/UQ:36842

Idioma(s)

eng

Publicador

Elsevier

Palavras-Chave #Biochemical Research Methods #Chemistry, Analytical #Mycophenolic Acid #Human Plasma #Glucuronide Metabolite #Transplant Recipients #Quantitative-analysis #Mofetil #Pharmacokinetics #Blood #Bioavailability #250400 Analytical Chemistry #C1 #730102 Immune system and allergy #320503 Clinical Pharmacology and Therapeutics
Tipo

Journal Article