Trypanosoma cruzi: Do different sylvatic strains trigger distinct immune responses?


Autoria(s): CAETANO, Leony Cristina; PRADO JR., Jose Clovis do; TOLDO, Miriam Paula Alonso; ABRAHAO, Ana Amelia Carraro
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Strains of Trypanosoma cruzi are multiclonal populations that can be classified in groups or genotypes, differing in pathogenicity, virulence, and histotropism. In this experiment the distinct behavior of two strains of T. cruzi, MORC-1 and MORC-2, was documented. Blood parasitemia, spleen proliferation, nitric oxide, histopathology of the spleen and heart were used as tools to evaluate parasite persistence. Groups of male mice were separated and divided in three groups: Control (C), Infected (IM-1) and Infected (IM-2). The peak of parasitemia occurred on 10 days post infection for both strains. LPS stimulated animals, infected MORC-2 group displayed significant higher concentrations of NO when compared to infected MORC-1 group (P < 0.05). For ConA stimulated lymphoproliferation, infected MORC-1 group displayed higher proliferation index as compared to infected MORC-2 group. An opposite behavior for IL-4 and TNF-alpha was observed according to the strain. For MORC-1 enhanced concentrations of IL-4 were present with concomitant reduced levels of TNF-alpha, while for MORC-2 enhanced concentrations of TNF-alpha and reduced levels of IL-4 were found. The histopathology of heart and spleen showed important differences in which MORC-1 displayed statistically enhanced number of amastigote in the heart and spleen as compared to MORC-2. Concluding, each strain triggered a distinct immune response with enhanced cytokine TH-1 profile for MORC-2 and TH-2 for MORC-1. (C) 2009 Elsevier Inc. All rights reserved.

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Identificador

EXPERIMENTAL PARASITOLOGY, v.124, n.2, p.219-224, 2010

0014-4894

http://producao.usp.br/handle/BDPI/20350

10.1016/j.exppara.2009.09.018

http://dx.doi.org/10.1016/j.exppara.2009.09.018

Idioma(s)

eng

Publicador

ACADEMIC PRESS INC ELSEVIER SCIENCE

Relação

Experimental Parasitology

Direitos

restrictedAccess

Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE

Palavras-Chave #Trypanosoma cruzi #Nitric oxide #Spleen proliferation #Histopathology #Mice #OXIDE-DEPENDENT MECHANISM #NECROSIS-FACTOR-ALPHA #NITRIC-OXIDE #GAMMA-INTERFERON #CHAGAS-DISEASE #T-CELLS #INFECTION #MICE #RESISTANCE #SUSCEPTIBILITY #Parasitology
Tipo

article

original article

publishedVersion