Strongyloides venezuelensis: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2008
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Resumo |
The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of U were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite. (C) 2008 Elsevier Inc. All rights reserved. |
Identificador |
EXPERIMENTAL PARASITOLOGY, v.119, n.1, p.7-14, 2008 0014-4894 http://producao.usp.br/handle/BDPI/20287 10.1016/j.exppara.2007.12.008 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
Experimental Parasitology |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #Strongyloides venezuelensis #strongyloidiasis #diagnosis #antigen #antibodies #IFAT #ELISA #immunoblot #LINKED-IMMUNOSORBENT-ASSAY #STERCORALIS INFECTION #POLYACRYLAMIDE GELS #DIAGNOSIS #IMMUNOFLUORESCENCE #ANTIBODIES #RATTI #ELISA #Parasitology |
Tipo |
article original article publishedVersion |