Strongyloides venezuelensis: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis


Autoria(s): MACHADO, Eleuza Rodrigues; FACCIOLI, Lucia Helena; COSTA-CRUZ, Julia Maria; LOURENCO, Elaine Vicente; ROQUE-BARREIRA, Maria Cristina; PIRES, Maria do Rosario de Fatima Goncalves; UETA, Marlene Tiduko
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of U were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite. (C) 2008 Elsevier Inc. All rights reserved.

Identificador

EXPERIMENTAL PARASITOLOGY, v.119, n.1, p.7-14, 2008

0014-4894

http://producao.usp.br/handle/BDPI/20287

10.1016/j.exppara.2007.12.008

http://dx.doi.org/10.1016/j.exppara.2007.12.008

Idioma(s)

eng

Publicador

ACADEMIC PRESS INC ELSEVIER SCIENCE

Relação

Experimental Parasitology

Direitos

restrictedAccess

Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE

Palavras-Chave #Strongyloides venezuelensis #strongyloidiasis #diagnosis #antigen #antibodies #IFAT #ELISA #immunoblot #LINKED-IMMUNOSORBENT-ASSAY #STERCORALIS INFECTION #POLYACRYLAMIDE GELS #DIAGNOSIS #IMMUNOFLUORESCENCE #ANTIBODIES #RATTI #ELISA #Parasitology
Tipo

article

original article

publishedVersion