BthMP: a new weakly hemorrhagic metalloproteinase from Bothrops moojeni snake venom


Autoria(s): GOMES, Mario Sergio R.; MENDES, Mirian M.; OLIVEIRA, Fabio de; ANDRADE, Rodrigo M. de; BERNARDES, Carolina P.; HAMAGUCHI, Amelia; ALCANTARA, Tania M. de; SOARES, Andreimar M.; RODRIGUES, Veridiana M.; HOMSI-BRANDEBURGO, Maria I.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

In this work, a new weakly hemorrhagic metalloproteinase (BthMP) was purified from Bothrops moojeni snake venom. This enzyme was homogeneous by native and SDS-PAGE. It showed a polypeptide chain of 23.5 kDa, pI=7.1, and N-terminal blocked. BthMP is comprised of high proteolytic activity on casein, fibrin and bovine fibrinogen, with no coagulating, esterase or phospholipase A(2) activities; it was inhibited by EDTA, EGTA and 1,10-phenanthroline and maintained its activity on pH from 7.0 to 9.0 and temperature from 5-40 degrees C. Assays with metal ions showed that Ca(2+) is an activator, whereas Zn(2+) and Hg(2+) inhibited about 50 and 80% of its activity, respectively. The edema evidenced the important role of the toxin in the inflammatory activity of the venom. BthMP also caused unclotting, and provoked histological alterations in the gastrocnemius muscle of mice inducing hemorrhage, necrosis and leukocytic infiltrate. The molecular mass and the inhibition assays suggest that the metal loproteinase BthMP belongs to class P-I of SVMPs. (c) 2008 Elsevier Ltd. All rights reserved.

Identificador

TOXICON, v.53, n.1, p.24-32, 2009

0041-0101

http://producao.usp.br/handle/BDPI/20244

10.1016/j.toxicon.2008.10.007

http://dx.doi.org/10.1016/j.toxicon.2008.10.007

Idioma(s)

eng

Publicador

PERGAMON-ELSEVIER SCIENCE LTD

Relação

Toxicon

Direitos

restrictedAccess

Copyright PERGAMON-ELSEVIER SCIENCE LTD

Palavras-Chave #Bothrops moojeni #Metalloproteinase #Snake venom #P-III METALLOPROTEINASE #BIOCHEMICAL-CHARACTERIZATION #FUNCTIONAL-CHARACTERIZATION #BOTHROPS-MOOJENI-(CAISSACA) VENOM #POLYACRYLAMIDE GELS #BASIC PROTEINASES #JARARHAGIN #LEUCURUS #PURIFICATION #JARARACA #Pharmacology & Pharmacy #Toxicology
Tipo

article

original article

publishedVersion