Mercury speciation in seafood samples by LC-ICP-MS with a rapid ultrasound-assisted extraction procedure: Application to the determination of mercury in Brazilian seafood samples
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2011
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Resumo |
This paper describes a simple method for mercury speciation in seafood samples by LC-ICP-MS with a fast sample preparation procedure. Prior to analysis, mercury species were extracted from food samples with a solution containing mercaptoethanol, L-cysteine and HCl and sonication for 15 min. Separation of mercury species was accomplished in less than 5 min on a C8 reverse phase column with a mobile phase containing 0.05%-v/v mercaptoethanol, 0.4% m/v L-cysteine and 0.06 mol L(-1) ammonium acetate. The method detection limits were found to be 0.25, 0.20 and 0.1 ng g(-1) for inorganic mercury, ethylmercury and methylmercury, respectively. Method accuracy is traceable to Certified Reference Materials (DOLT-3 and DORM-3) from the National Research Council Canada (NRCC). With the proposed method there is a considerable reduction of the time of sample preparation. Finally, the method was applied for the speciation of mercury in seafood samples purchased from the Brazilian market. (C) 2010 Elsevier Ltd. All rights reserved. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) |
Identificador |
FOOD CHEMISTRY, v.126, n.4, p.2000-2004, 2011 0308-8146 http://producao.usp.br/handle/BDPI/20231 10.1016/j.foodchem.2010.12.068 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCI LTD |
Relação |
Food Chemistry |
Direitos |
restrictedAccess Copyright ELSEVIER SCI LTD |
Palavras-Chave | #ICP-MS #Speciation #Food samples #Mercury #Sample preparation #Methylmercury #Liquid chromatography #PLASMA-MASS SPECTROMETRY #SPECIES TRANSFORMATIONS #BIOLOGICAL SAMPLES #INTERNAL STANDARD #HUMAN HAIR #GC-ICPMS #FISH #METHYLMERCURY #DIGESTION #ELEMENTS #Chemistry, Applied #Food Science & Technology #Nutrition & Dietetics |
Tipo |
article original article publishedVersion |