Mercury speciation in seafood samples by LC-ICP-MS with a rapid ultrasound-assisted extraction procedure: Application to the determination of mercury in Brazilian seafood samples


Autoria(s): BATISTA, Bruno Lemos; RODRIGUES, Jairo L.; SOUZA, Samuel S. de; SOUZA, Vanessa C. Oliveira; BARBOSA JR., Fernando
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2011

Resumo

This paper describes a simple method for mercury speciation in seafood samples by LC-ICP-MS with a fast sample preparation procedure. Prior to analysis, mercury species were extracted from food samples with a solution containing mercaptoethanol, L-cysteine and HCl and sonication for 15 min. Separation of mercury species was accomplished in less than 5 min on a C8 reverse phase column with a mobile phase containing 0.05%-v/v mercaptoethanol, 0.4% m/v L-cysteine and 0.06 mol L(-1) ammonium acetate. The method detection limits were found to be 0.25, 0.20 and 0.1 ng g(-1) for inorganic mercury, ethylmercury and methylmercury, respectively. Method accuracy is traceable to Certified Reference Materials (DOLT-3 and DORM-3) from the National Research Council Canada (NRCC). With the proposed method there is a considerable reduction of the time of sample preparation. Finally, the method was applied for the speciation of mercury in seafood samples purchased from the Brazilian market. (C) 2010 Elsevier Ltd. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Identificador

FOOD CHEMISTRY, v.126, n.4, p.2000-2004, 2011

0308-8146

http://producao.usp.br/handle/BDPI/20231

10.1016/j.foodchem.2010.12.068

http://dx.doi.org/10.1016/j.foodchem.2010.12.068

Idioma(s)

eng

Publicador

ELSEVIER SCI LTD

Relação

Food Chemistry

Direitos

restrictedAccess

Copyright ELSEVIER SCI LTD

Palavras-Chave #ICP-MS #Speciation #Food samples #Mercury #Sample preparation #Methylmercury #Liquid chromatography #PLASMA-MASS SPECTROMETRY #SPECIES TRANSFORMATIONS #BIOLOGICAL SAMPLES #INTERNAL STANDARD #HUMAN HAIR #GC-ICPMS #FISH #METHYLMERCURY #DIGESTION #ELEMENTS #Chemistry, Applied #Food Science & Technology #Nutrition & Dietetics
Tipo

article

original article

publishedVersion