Antileukemic effects of Didemnum psammatodes (Tunicata: Ascidiacea) constituents


Autoria(s): TAKEARA, Renata; JIMENEZ, Paula Christine; WILKE, Diego Veras; MORAES, Manoel Odorico de; PESSOA, Claudia; LOPES, Norberto Peporine; LOPES, Joao Luis Callegari; LOTUFO, Tito Monteiro da Cruz; COSTA-LOTUFO, Leticia Veras
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Chemical investigation of the methanolic extract of the ascidian Didemnum psammatodes has led to the identification of fourteen known compounds: three methyl esters (methyl myristate, methyl palmitate and methyl stearate), four steroids (cholesterol, campesterol, stigmasterol and beta-sitosterol), two fatty acids (palmitic acid and stearic acid), three glyceryl ethers {(1,2-propanediol, 3-(heptadecyloxy), batyl alcohol and 1,2-propanediol, 3-[(methyloctadecyl)oxy]) and two nucleosides (thymidine and 2`-deoxyguanosine). Their structures were proposed by NMR and comparison with literature data and GC analysis in comparison with authentic sample. The cytotoxic activity of these compounds was evaluated against human leukemia cell line panel using the MTT assay. The mixture of the three methyl esters was the most active group of compounds, showing antiproliferative and cytotoxic effects. Further studies on their mode of action suggest that these activities are connected with inhibition of DNA synthesis and induction of both necrosis and apoptosis. (C) 2007 Elsevier Inc. All rights reserved.

Identificador

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v.151, n.3, Special Issue, p.363-369, 2008

1095-6433

http://producao.usp.br/handle/BDPI/19924

10.1016/j.cbpa.2007.02.011

http://dx.doi.org/10.1016/j.cbpa.2007.02.011

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE INC

Relação

Comparative Biochemistry and Physiology A-molecular & Integrative Physiology

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE INC

Palavras-Chave #Chemical constituent #Cytotoxicity #Didemnum psammatodes #Human leukemia cell lines #Tunicate #CHEMICAL-COMPOSITION #LAMELLARIN ALKALOIDS #CYCLIC HEPTAPEPTIDE #CANARY-ISLANDS #CELLS #MOLLE #ACID #SPONGES #PROLIFERATION #METABOLITES #Biochemistry & Molecular Biology #Physiology #Zoology
Tipo

article

original article

publishedVersion