Immunogenicity of a Whole-Cell Pertussis Vaccine with Low Lipopolysaccharide Content in Infants


Autoria(s): ZORZETO, Tatiane Queiroz; HIGASHI, Hisako Gondo; SILVA, Marcos Tadeu Nolasco da; CARNIEL, Emilia de Faria; DIAS, Waldely Oliveira; RAMALHO, Vanessa Domingues; MAZZOLA, Tais Nitsch; LIMA, Simone Corte Batista Souza; MORCILLO, Andre Moreno; STEPHANO, Marco Antonio; ANTONIO, Maria Angela Reis de Goes; ZANOLLI, Maria de Lurdes; RAW, Isaias; VILELA, Maria Marluce dos Santos
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wP(low) vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wP(low) vaccine. Proliferation of CD3(+) T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3(+), CD4(+), CD8(+), and T-cell receptor gamma delta-positive (gamma delta(+)) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in super-natants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3(+) blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wP(low) vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wP(low) vaccine; P = 0.029). The frequencies of proliferating CD4(+), CD8(+), and gamma delta(+) cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of gamma delta(+) cells in the B. pertussis cultures (P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3(+) cell proliferation, and gamma delta(+) cell expansions were similar with the two vaccines.

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Sao Paulo, Brazil[2005/03539-1]

Financiadora de Estudos e Projetos (FINEP), Brazil[01040957/0]

Identificador

CLINICAL AND VACCINE IMMUNOLOGY, v.16, n.4, p.544-550, 2009

1556-6811

http://producao.usp.br/handle/BDPI/19789

10.1128/CVI.00339-08

http://dx.doi.org/10.1128/CVI.00339-08

Idioma(s)

eng

Publicador

AMER SOC MICROBIOLOGY

Relação

Clinical and Vaccine Immunology

Direitos

restrictedAccess

Copyright AMER SOC MICROBIOLOGY

Palavras-Chave #GAMMA/DELTA T-CELLS #BORDETELLA-PERTUSSIS #MEDIATED-IMMUNITY #PROTECTIVE IMMUNITY #ACELLULAR VACCINES #ANTIBODY-RESPONSES #CONTROLLED TRIAL #INFECTION #CHILDREN #EFFICACY #Immunology #Infectious Diseases #Microbiology
Tipo

article

original article

publishedVersion