A New Approach to Ammonium Sulphate Feeding for Fed-Batch Arthrospira (Spirulina) platensis Cultivation in Tubular Photobioreactor


Autoria(s): FERREIRA, Livia S.; RODRIGUES, Mayla S.; CONVERTI, Attilio; Sato, Sunao; CARVALHO, Joao Carlos M.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Arthrospira platensis was cultivated in tubular photobioreactor using different photosynthetic photon flux densities (PPFD) and protocols of (NH(4))(2)SO(4) fed-hatch supply. Results were evaluated by variance analysis selecting maximum cell concentration (X(m)), cell productivity (P(x)), nitrogen-to-cell conversion factor (Y(X/N)) and biomass, protein and lipid contents as responses. At PPFD of 120 and 240 mu mol-photons/m(2) s, a parabolic profile of (NH(4))(2)SO(4) addition aiming at producing biomass with 7% nitrogen content ensured X(m) values (14.1 and 12.2 g/L, respectively) comparable to those obtained with NaNO(3). At PPFD of 240 mu mol-photons/m(2) s, P(x) (1.69 g/Ld) was 36% higher, although the photosynthetic efficiency (3.0%) was less than one-half that at PPFD of 120 mu mol-photons/m(2) s. Biomass was shown to be constituted by about 35% proteins and 10% lipids, without any dependence on PPFD or kind of nitrogen source. These results highlight the possible use of (NH(4))(2)SO(4) as alternative, cheap nitrogen source for A. platensis cultivation in tubular photobioreactors. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 1271-1277, 2010

CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)

FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo), Sao Paulo - Brazil

Identificador

BIOTECHNOLOGY PROGRESS, v.26, n.5, p.1271-1277, 2010

8756-7938

http://producao.usp.br/handle/BDPI/19757

10.1002/btpr.457

http://dx.doi.org/10.1002/btpr.457

Idioma(s)

eng

Publicador

JOHN WILEY & SONS LTD

Relação

Biotechnology Progress

Direitos

closedAccess

Copyright JOHN WILEY & SONS LTD

Palavras-Chave #Arthrospira (Spirulina) platensis #fed-batch process #ammonium sulphate #photosynthetic photon flux density #tubular photobioreactor #LIGHT-INTENSITY #NITROGEN-SOURCE #MICROALGAE #TEMPERATURE #CULTURE #UREA #CHLORIDE #GROWTH #CHLOROPHYLL #EFFICIENCY #Biotechnology & Applied Microbiology #Food Science & Technology
Tipo

article

original article

publishedVersion