Rapid Determination of Water-Soluble and Fat-Soluble Vitamins in Commercial Formulations by MEEKC
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2010
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Resumo |
Microemulsion electrokinetic capillary chromatography has been successfully applied to the separation and determination of water-soluble vitamins (thiamine hydrochloride, riboflavin, niacin, pyridoxine hydrochloride, folic acid, cobalamin, ascorbic acid) and a fat-soluble vitamin (alpha-tocopherol acetate). The optimal microemulsion buffer contained sodium dodecylsulfate (SDS) as surfactant, butan-1-ol as the co-surfactant, ethyl acetate as the oil and pH 9.2 tetraborate buffer, modified with 15% (v/v) 2-propanol. UV detection at 214 nm gave adequate sensitivity without interference from sample excipients. Under the optimized conditions, the vitamins were baseline separated in less than 7 min. Analytical curves of peak area versus concentration presented coefficients of determination (R (2) ) > 0.99, acceptable limits of quantification between 8.40 and 16.23 mu g mL(-1) were obtained. Vitamin levels in liquid formulation were quantified with intra-day precision better than 0.99% RSD for migration time and 1.19% RSD for peak area ratio. Recoveries ranged between 98.7 and 101.7%. The method was considered appropriate for rapid and routine analysis. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo of Brazil (FAPESP)[00/04414-4] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo of Brazil (FAPESP)[02/07390-4] |
Identificador |
CHROMATOGRAPHIA, v.72, n.7/Ago, p.687-694, 2010 0009-5893 http://producao.usp.br/handle/BDPI/19659 10.1365/s10337-010-1704-9 |
Idioma(s) |
eng |
Publicador |
SPRINGER HEIDELBERG |
Relação |
Chromatographia |
Direitos |
restrictedAccess Copyright SPRINGER HEIDELBERG |
Palavras-Chave | #Microemulsion electrokinetic chromatography #Fat-soluble vitamins #Water-soluble vitamins #MICROEMULSION ELECTROKINETIC CHROMATOGRAPHY #ELECTROOSMOTIC FLOW ENVIRONMENT #CAPILLARY CHROMATOGRAPHY #PHARMACEUTICAL ANALYSIS #ALPHA-TOCOPHEROL #DELTA-TOCOPHEROL #WIDE-RANGE #SEPARATION #OPTIMIZATION #MICELLAR #Biochemical Research Methods #Chemistry, Analytical |
Tipo |
article original article publishedVersion |