Rapid Determination of Water-Soluble and Fat-Soluble Vitamins in Commercial Formulations by MEEKC


Autoria(s): AURORA-PRADO, Maria S.; SILVA, Claudinei A.; TAVARES, Marina F. M.; ALTRIA, Kevin D.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2010

Resumo

Microemulsion electrokinetic capillary chromatography has been successfully applied to the separation and determination of water-soluble vitamins (thiamine hydrochloride, riboflavin, niacin, pyridoxine hydrochloride, folic acid, cobalamin, ascorbic acid) and a fat-soluble vitamin (alpha-tocopherol acetate). The optimal microemulsion buffer contained sodium dodecylsulfate (SDS) as surfactant, butan-1-ol as the co-surfactant, ethyl acetate as the oil and pH 9.2 tetraborate buffer, modified with 15% (v/v) 2-propanol. UV detection at 214 nm gave adequate sensitivity without interference from sample excipients. Under the optimized conditions, the vitamins were baseline separated in less than 7 min. Analytical curves of peak area versus concentration presented coefficients of determination (R (2) ) > 0.99, acceptable limits of quantification between 8.40 and 16.23 mu g mL(-1) were obtained. Vitamin levels in liquid formulation were quantified with intra-day precision better than 0.99% RSD for migration time and 1.19% RSD for peak area ratio. Recoveries ranged between 98.7 and 101.7%. The method was considered appropriate for rapid and routine analysis.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo of Brazil (FAPESP)[00/04414-4]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo of Brazil (FAPESP)[02/07390-4]

Identificador

CHROMATOGRAPHIA, v.72, n.7/Ago, p.687-694, 2010

0009-5893

http://producao.usp.br/handle/BDPI/19659

10.1365/s10337-010-1704-9

http://dx.doi.org/10.1365/s10337-010-1704-9

Idioma(s)

eng

Publicador

SPRINGER HEIDELBERG

Relação

Chromatographia

Direitos

restrictedAccess

Copyright SPRINGER HEIDELBERG

Palavras-Chave #Microemulsion electrokinetic chromatography #Fat-soluble vitamins #Water-soluble vitamins #MICROEMULSION ELECTROKINETIC CHROMATOGRAPHY #ELECTROOSMOTIC FLOW ENVIRONMENT #CAPILLARY CHROMATOGRAPHY #PHARMACEUTICAL ANALYSIS #ALPHA-TOCOPHEROL #DELTA-TOCOPHEROL #WIDE-RANGE #SEPARATION #OPTIMIZATION #MICELLAR #Biochemical Research Methods #Chemistry, Analytical
Tipo

article

original article

publishedVersion