(1)H NMR determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental and biological samples
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
19/10/2012
19/10/2012
2009
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Resumo |
A nuclear magnetic resonance ((1)H NMR) method for the determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental aqueous samples was developed and validated. L-BMAA is a neurotoxic modified amino acid that can be produced by cyanobacteria in aqueous environments. This toxin was extracted from samples by means of solid-phase extraction (SPE) and identified and quantified by (1)H NMR without further derivatization steps. The lower limit of quantification (LLOQ) was 5 mu g/mL Good inter and intra-assay precision was also observed (relative standard deviation <8.5%) with the use of 4-nitro-DL-phenylalanine as an internal standard (IS). This method of 1H NMR analysis is not time consuming and can be readily utilized to monitor L-BMAA and confirm its presence in environmental and biological samples. (C) 2008 Elsevier Ltd. All rights reserved. Conselho Nacional de Desenvolvimento e Technologia (CNPq) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) |
Identificador |
TOXICON, v.53, n.5, p.578-583, 2009 0041-0101 http://producao.usp.br/handle/BDPI/19624 10.1016/j.toxicon.2008.12.013 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD |
Relação |
Toxicon |
Direitos |
restrictedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #L-BMAA #(1)H NMR #Environmental samples #Biological samples #SPE #NEUROTOXIC AMINO-ACID #CYANOBACTERIAL NEUROTOXINS #NEURODEGENERATIVE DISEASE #LIQUID-CHROMATOGRAPHY #MASS-SPECTROMETRY #GUAM #SPECTROSCOPY #DERIVATIVES #DILUTE #Pharmacology & Pharmacy #Toxicology |
Tipo |
article original article publishedVersion |