(1)H NMR determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental and biological samples


Autoria(s): MOURA, Sidnei; ULTRAMARI, Mariah de Almeida; PAULA, Daniela Mendes Louzada de; YONAMINE, Mauricio; PINTO, Ernani
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2009

Resumo

A nuclear magnetic resonance ((1)H NMR) method for the determination of beta-N-methylamino-L-alanine (L-BMAA) in environmental aqueous samples was developed and validated. L-BMAA is a neurotoxic modified amino acid that can be produced by cyanobacteria in aqueous environments. This toxin was extracted from samples by means of solid-phase extraction (SPE) and identified and quantified by (1)H NMR without further derivatization steps. The lower limit of quantification (LLOQ) was 5 mu g/mL Good inter and intra-assay precision was also observed (relative standard deviation <8.5%) with the use of 4-nitro-DL-phenylalanine as an internal standard (IS). This method of 1H NMR analysis is not time consuming and can be readily utilized to monitor L-BMAA and confirm its presence in environmental and biological samples. (C) 2008 Elsevier Ltd. All rights reserved.

Conselho Nacional de Desenvolvimento e Technologia (CNPq)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

Identificador

TOXICON, v.53, n.5, p.578-583, 2009

0041-0101

http://producao.usp.br/handle/BDPI/19624

10.1016/j.toxicon.2008.12.013

http://dx.doi.org/10.1016/j.toxicon.2008.12.013

Idioma(s)

eng

Publicador

PERGAMON-ELSEVIER SCIENCE LTD

Relação

Toxicon

Direitos

restrictedAccess

Copyright PERGAMON-ELSEVIER SCIENCE LTD

Palavras-Chave #L-BMAA #(1)H NMR #Environmental samples #Biological samples #SPE #NEUROTOXIC AMINO-ACID #CYANOBACTERIAL NEUROTOXINS #NEURODEGENERATIVE DISEASE #LIQUID-CHROMATOGRAPHY #MASS-SPECTROMETRY #GUAM #SPECTROSCOPY #DERIVATIVES #DILUTE #Pharmacology & Pharmacy #Toxicology
Tipo

article

original article

publishedVersion