Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
19/10/2012
19/10/2012
2010
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| Resumo |
In this work we report on a study of the morphological changes of LDL induced in vitro by metallic ions (Cu(2+) and Fe(3+)). These modifications were characterized by transmission electron microscopy, nuclear magnetic resonance and the Z-scan technique. The degree of oxidative modification of LDL was determined by the TBARS and lipid hydroperoxides assays. It is shown that distinct pathways for modifying lipoproteins lead to different morphological transformations of the particles characterized by changes in size and/or shape of the resulting particles, and by the tendency to induce aggregation of the particles. There were no evidence of melting of particles promoted by oxidative processes with Cu and Fe. (C) 2010 Elsevier Ireland Ltd. All rights reserved. State of Sao Paulo Research Foundation (FAPESP) National Institute of Science and Technology of Complex Fluids (INCT-FCx) National Counsel of Technological and Scientific Development (CNPq) |
| Identificador |
CHEMISTRY AND PHYSICS OF LIPIDS, v.163, n.6, p.545-551, 2010 0009-3084 http://producao.usp.br/handle/BDPI/19578 10.1016/j.chemphyslip.2010.03.008 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER IRELAND LTD |
| Relação |
Chemistry and Physics of Lipids |
| Direitos |
restrictedAccess Copyright ELSEVIER IRELAND LTD |
| Palavras-Chave | #oxLDL #Lipoprotein #NMR #TEM #Z-scan technique #LIPID-PEROXIDATION #ATHEROSCLEROTIC LESIONS #OXIDIZED LIPOPROTEINS #SERUM COPPER #MODIFIED LDL #FUSION #ATHEROGENESIS #ANTIOXIDANTS #AGGREGATION #IDENTIFICATION #Biochemistry & Molecular Biology #Biophysics |
| Tipo |
article original article publishedVersion |
