Evaluation of nutritional and genetic determinants of total homocysteine, methylmalonic acid and S-adenosylmethionine/S-adenosylhomocysteine values in Brazilian childbearing-age women


Autoria(s): BARBOSA, Patricia R.; STABLER, Sally P.; TRENTIN, Renata; CARVALHO, Felipe R.; LUCHESSI, Andre D.; HIRATA, Rosario D. C.; HIRATA, Mario H.; ALLEN, Robert H.; GUERRA-SHINOHARA, Elvira M.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/10/2012

19/10/2012

2008

Resumo

Background: Cobalamin (Cbl) and folate deficiencies and gene polymorphism of key enzymes or carriers can impair homocysteine metabolism and may change the serum values of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). We investigated the nutritional and genetic determinants for total homocysteine (tHcy), methylmalonic acid (MMA) and SAM/SAH in healthy Brazilian childbearing-age women. Methods: Serum concentrations of Cbl, folate, red blood cell folate, ferritin, tHcy, MMA, SAM, SAH and other metabolites were measured in 102 healthy unrelated women. The genotypes for MTHFR C677T, MTHFR A1298C, MTR A2756G, MTRR A66G, TC2 C776G, TC2 A67G and RFCI A80G gene polymorphisms were identified by PCR-RFLP. Results: Serum folate and Cbl were inversely correlated with tHcy and serum MMA, respectively. Cbl deficiency was associated with increased MMA and reduced alpha-aminobutyrate, serine and N-methylglycine concentrations. No variable was associated with SAM/SAH ratio. In addition, gene polymorphisms were not selected as determinants for tHcy, MMA and SAM/SAH ratio. Iron, Cbl and folate deficiencies were found respectively in 30.4%, 22.5% and 2.0% of individuals studied. Conclusions: There was a high frequency of Cbl and iron deficiency in this group of childbearing-age women. Serum folate and Cbl were the determinants of serum tHcy and MMA concentration, respectively. (c) 2007 Elsevier B.V. All rights reserved.

Identificador

CLINICA CHIMICA ACTA, v.388, n.1/Fev, p.139-147, 2008

0009-8981

http://producao.usp.br/handle/BDPI/19552

10.1016/j.cca.2007.10.023

http://dx.doi.org/10.1016/j.cca.2007.10.023

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Clinica Chimica Acta

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #cobalamin #folate #polymorphisms #homocysteine #methylmalonic acid #women #NEURAL-TUBE DEFECTS #METHIONINE SYNTHASE REDUCTASE #REDUCED FOLATE CARRIER #CHROMATOGRAPHY-MASS-SPECTROMETRY #GLUTAMATE CARBOXYPEPTIDASE-II #METHYLENETETRAHYDROFOLATE REDUCTASE #PLASMA HOMOCYSTEINE #SPINA-BIFIDA #COBALAMIN DEFICIENCY #TRANSCOBALAMIN GENE #Medical Laboratory Technology
Tipo

article

original article

publishedVersion