Genetic Transformation of Passionflower and Evaluation of R(1) and R(2) Generations for Resistance to Cowpea aphid borne mosaic virus


Autoria(s): MONTEIRO-HARA, Alessandra C. B. A.; JADAO, Adriana S.; MENDES, Beatriz M. J.; REZENDE, Jorge A. M.; TREVISAN, Flavio; MELLO, Ana Paula O. A.; VIEIRA, Maria Lucia C.; MELETTI, L. M. M.; PIEDADE, Sonia M. De S.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2011

Resumo

We report on the production and evaluation of passionflower transgenic lines for resistance to Cowpea aphid borne mosaic virus (CABMV). Genetic transformation was done using Agrobacterium tumefaciens and transgene integration was confirmed by Southern blot analyses, resulting in nine transgenic lines for `IAC 275` and three for `IAC 277`. Transgenic lines were clonally propagated and evaluated for resistance to CABMV After the third inoculation, under higher inoculum pressure, only propagated plants of the transgenic line T16 remained asymptomatic, indicating a high resistance to infection with CABMV. This transgenic line was self-pollinated and the RI generation was evaluated together with the RI generation of another resistant transgenic line (T2) identified previously. Plants were inoculated with CABMV by means of viruliferous Myzus nicotianae. All 524 T2R(1) plants became infected, whereas 13 of 279 T16R(1) remained asymptomatic after four successive inoculations. A TI6R(2) generation was obtained and plants were inoculated with CABMV mechanically or by aphids. After successive inoculations, 118 of 258 plants were symptomless, suggesting that the resistance to CABMV was maintained in the plant genome as the homozygous condition was achieved. Five selected resistant TI6R(2) plants which contained the capsid protein gene are being crossed for further analyses.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES/PNPD)

Identificador

PLANT DISEASE, v.95, n.8, p.1021-1025, 2011

0191-2917

http://producao.usp.br/handle/BDPI/19360

10.1094/PDIS-12-10-0873

http://dx.doi.org/10.1094/PDIS-12-10-0873

Idioma(s)

eng

Publicador

AMER PHYTOPATHOLOGICAL SOC

Relação

Plant Disease

Direitos

restrictedAccess

Copyright AMER PHYTOPATHOLOGICAL SOC

Palavras-Chave #IN-VITRO ORGANOGENESIS #PAPAYA-RINGSPOT-VIRUS #MEDIATED RESISTANCE #F-FLAVICARPA #COAT PROTEIN #PASSIFLORA #PASSIONFRUIT #DISEASE #PLANTS #Plant Sciences
Tipo

article

original article

publishedVersion