Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
18/10/2012
18/10/2012
2008
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Resumo |
We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems. |
Identificador |
JOURNAL OF FLUORESCENCE, v.18, n.1, p.169-174, 2008 1053-0509 http://producao.usp.br/handle/BDPI/18764 10.1007/s10895-007-0260-7 |
Idioma(s) |
eng |
Publicador |
SPRINGER/PLENUM PUBLISHERS |
Relação |
Journal of Fluorescence |
Direitos |
restrictedAccess Copyright SPRINGER/PLENUM PUBLISHERS |
Palavras-Chave | #cholesterol #europium #fluorescence #optical spectroscopy #HYDROGEN-PEROXIDE #FLUORESCENT-PROBE #LUMINESCENCE #BIOSENSOR #ELECTRODE #GLUCOSE #OXIDASE #Biochemical Research Methods #Chemistry, Analytical |
Tipo |
article original article publishedVersion |