Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol


Autoria(s): SILVA, Flavia Rodrigues de Oliveira; SAMAD, Ricardo Elgul; GOMES, Laercio; COURROL, Lilia Coronato
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2008

Resumo

We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.

Identificador

JOURNAL OF FLUORESCENCE, v.18, n.1, p.169-174, 2008

1053-0509

http://producao.usp.br/handle/BDPI/18764

10.1007/s10895-007-0260-7

http://dx.doi.org/10.1007/s10895-007-0260-7

Idioma(s)

eng

Publicador

SPRINGER/PLENUM PUBLISHERS

Relação

Journal of Fluorescence

Direitos

restrictedAccess

Copyright SPRINGER/PLENUM PUBLISHERS

Palavras-Chave #cholesterol #europium #fluorescence #optical spectroscopy #HYDROGEN-PEROXIDE #FLUORESCENT-PROBE #LUMINESCENCE #BIOSENSOR #ELECTRODE #GLUCOSE #OXIDASE #Biochemical Research Methods #Chemistry, Analytical
Tipo

article

original article

publishedVersion