Aqueous two-phase extraction using thermoseparating copolymer: a new system for phenolic compounds removal from hemicelullosic hydrolysate


Autoria(s): HASMANN, Francislene A.; SANTOS, Valeria C.; GURPILHARES, Daniela B.; PESSOA-JUNIOR, Adalberto; ROBERTO, Ines C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2008

Resumo

BACKGROUND: The hydrolysis of hemicellulosic material can provide liquor with high xylose concentration (which can be used as a fermentation medium) and phenolic compounds (Phs), potentially immunostimulating compounds. However, these hydrolysates must be detoxified in order to remove the Phs that can act as inhibitors in bioconversions. RESULTS: Aqueous two-phase systems composed of thermoseparating copolymers were used for rice straw hydrolysate detoxification. The hydrolysis process was able to promote chemical breakdown of 85% of the total hemicellulose content, 14% of the cellulose, and 2% of the lignin. The hydrolysate obtained contained 19.7 g L-1 of xylose and several phenolic compounds, such as vanillin, vanillic acid, ferullic acid, etc. The phenolics extraction was studied as a function of copolymer molar mass (1100 g mol(-1), 2000 g mol(-1) and 2800 g mol(-1)), their percentages (from 5% to 50%) and Phs initial concentration. Phenolic compounds extraction of around 80% was obtained under the following conditions: 20% (w/w) and 35% (w/w) copolymer 1100 g mol-1, 35% (w/w) copolymer 2000 g mol(-1) and 35% (w/w) copolymer 2800 g mol(-1) at 25 degrees C. CONCLUSIONS: The results demonstrated the viability of this method for the removal of Phs from rice straw hydrolysate, which has potential uses in bioconversion processes. (c) 2007 Society of Chemical Industry.

Identificador

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v.83, n.2, p.167-173, 2008

0268-2575

http://producao.usp.br/handle/BDPI/17643

10.1002/jctb.1779

http://dx.doi.org/10.1002/jctb.1779

Idioma(s)

eng

Publicador

JOHN WILEY & SONS LTD

Relação

Journal of Chemical Technology and Biotechnology

Direitos

restrictedAccess

Copyright JOHN WILEY & SONS LTD

Palavras-Chave #aqueous two-phase systems #recovery #thermoseparating #triblock copolymer #residues #phenolics compounds #XYLITOL PRODUCTION #PURIFICATION #FERMENTATION #TEMPERATURE #ACID #DETOXIFICATION #PROTEIN #XYLOSE #Biotechnology & Applied Microbiology #Chemistry, Multidisciplinary #Engineering, Chemical
Tipo

article

original article

publishedVersion