Characterization of commercial cellulases and their use in the saccharification of a sugarcane bagasse sample pretreated with dilute sulfuric acid


Autoria(s): SANTOS, Victor T. O.; ESTEVES, Paula J.; Milagres, Adriane Maria Ferreira; CARVALHO, Walter
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2011

Resumo

This study aimed to correlate the efficiency of enzymatic hydrolysis of the cellulose contained in a sugarcane bagasse sample pretreated with dilute H(2)SO(4) with the levels of independent variables such as initial content of solids and loadings of enzymes and surfactant (Tween 20), for two cellulolytic commercial preparations. The preparations, designated cellulase I and cellulase II, were characterized regarding the activities of total cellulases, endoglucanase, cellobiohydrolase, cellobiase, beta-glucosidase, xylanase, and phenoloxidases (laccase, manganese and lignin peroxidases), as well as protein contents. Both extracts showed complete cellulolytic complexes and considerable activities of xylanases, without activities of phenoloxidases. For the enzymatic hydrolyses, two 2(3) central composite full factorial designs were employed to evaluate the effects caused by the initial content of solids (1.19-4.81%, w/w) and loadings of enzymes (1.9-38.1 FPU/g bagasse) and Tween 20 (0.0-0.1 g/g bagasse) on the cellulose digestibility. Within 24 h of enzymatic hydrolysis, all three independent variables influenced the conversion of cellulose by cellulase I. Using cellulase II, only enzyme and surfactant loadings showed significant effects on cellulose conversion. An additional experiment demonstrated the possibility of increasing the initial content of solids to values much higher than 4.81% (w/w) without compromising the efficiency of cellulose conversion, consequently improving the glucose concentration in the hydrolysate.

FAPESP

CNPq

CAPES

Identificador

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.38, n.8, p.1089-1098, 2011

1367-5435

http://producao.usp.br/handle/BDPI/17607

10.1007/s10295-010-0888-1

http://dx.doi.org/10.1007/s10295-010-0888-1

Idioma(s)

eng

Publicador

SPRINGER HEIDELBERG

Relação

Journal of Industrial Microbiology & Biotechnology

Direitos

restrictedAccess

Copyright SPRINGER HEIDELBERG

Palavras-Chave #Enzymatic hydrolysis #Statistical design #Surfactant #Fermentable sugars #ENZYMATIC-HYDROLYSIS #PHANEROCHAETE-CHRYSOSPORIUM #LIGNOCELLULOSIC BIOMASS #CELLULOSE HYDROLYSIS #MANGANESE PEROXIDASE #TRICHODERMA-REESEI #ASPERGILLUS-NIGER #CORN STOVER #LIGNIN #LACCASE #Biotechnology & Applied Microbiology
Tipo

article

original article

publishedVersion